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[RiDC] gp32 sequence doesn't match sequencing results #1

Open fxbuson opened 6 months ago

fxbuson commented 6 months ago

After getting sequencing results for the gp32 gene in the Research in Diagnostics Collection, it turned out that the actual sequence in the CDS DNA is very different. It's also missing a His tag. It does translate to the same amino acids, so I imagine there was some confusion between codon optimized versions.

Here is the C-terminal sequence I got from my sequencing results:

GGGAAACCCCCGTTGATGTTACGTGCCCTTGGGAAGGTGCAAACTTTGTCTTGAAAGTTAAGCAAGTATCTGGATTTAGTAACTATGACGAGTCAAAGTTTCTTAATCAATCAGCGATTCCAAACATTGACGATGAGTCATTCCAAAAAGAACTGTTTGAGCAAATGGTTGATTTATCGGAGATGACCAGTAAAGACAAATTTAAGAGTTTCGAGGAACTTAATACTAAGTTCGGACAAGTAATGGGAACCGCCGTAATGGGCGGTGCAGCGGCAACCGCTGCAAAGAAAGCAGATAAAGTCGCCGACGATTTGGATGCATTTAACGTTGACGATTTCAATACGAAAACCGAGGATGACTTCATGAGTAGCTCGTCCGGCTCTAGCAGCTCAGCGGACGATACAGACTTAGATGATTTACTGAATGATCTGTAATAA