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RobertsLab / paper-Olurida-gene

Gene expression data and R scripts for analysis
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Explain/clarify qPCR analysis #8

Closed kubu4 closed 7 years ago

kubu4 commented 7 years ago

Please explicitly state why the normalized mRNA levels are calculated as ΔCt= (target Ct – actin Ct) as well as why a logΔCt were used for statistical analysis. In the results section the y-axis is labeled as the data being plotted at a ΔCt value. The statistical calculations being performed on logΔCt value but the graphs showing just the ΔCt can be a bit confusing. It’s not so much that there’s an inherent problem with how the data were analyzed, but rather just being clear why it was done this way.

sr320 commented 7 years ago

Confer with Brent - but my recall is log was used to better meet assumptions of stat test... Normalization

kubu4 commented 7 years ago

Updated text and revised to reflect use of logΔCt throughout. Figures need updating - created separate issue #12 .