RobertsLab / project-oyster-oa

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Source of blib? #11

Closed sr320 closed 6 years ago

sr320 commented 6 years ago

What is 2017-05-23-oyster-desearleinated.blib ?

Where did it come from.

place short explanation on protocol

https://github.com/RobertsLab/project-oyster-oa/blob/master/notebooks/DNR/2017-09-28-SRM-Skyline-Data-Pipeline.ipynb

yaaminiv commented 6 years ago

From my lab notebook:

Emma and her team were able to figure out that brecan (a version of pecanpie produced by Brian Searle in Genome Sciences) “produces blib files that have a certain number of incompatibilities with Skyline (one of which is redundant peptide identifications). Jarrett Egertson wrote a script to get rid of these incompatibilities so that the brecan blib files work,” (Timmins-Schiffman, pers. comm.)

Essentially Emma made my original .blib while I was going through my DIA analyses and I found that there were extremely high error rates in my Skyline document. So someone fixed the problem and created a new .blib using a version of PECAN. I don't have the script but can ask for it.

sr320 commented 6 years ago

again be sure to add in protocol

yaaminiv commented 6 years ago

Will do.

yaaminiv commented 6 years ago

Protocol updated accordingly

screen shot 2017-10-10 at 2 14 04 pm