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ID oyster diploid/triploid families #1689

Open sr320 opened 1 year ago

sr320 commented 1 year ago

Some pics from PW...

image

There were 4 bags in one upweller. we color coded the four bags...

image image image image image

@AHuffmyer might have more useful info to add

Blues were clearly biggest and had the largest numbers

AHuffmyer commented 1 year ago

No notes to add - sizes and shell colors were pretty variable in the other bags, so there were no clear phenotype differences.

mattgeorgephd commented 1 year ago

I took a few photos of the upwellers when I placed the bags in there. Here is a presentation with the pictures and my best guesses as to which families are in which. As memory also serves, there were not very many F5 3n, but they were big!

sr320 commented 1 year ago

we only dealt with outside tanks -- did not know any are / were inside...

note F14 (2n) were outside

We have now know cohorts (bags) as

Orange Pink Blue Yellow

thus

Yellow = F14 (2n)

My take is we should have 8 bags but only have 4? @mattgeorgephd sorry but missed your best guesses?

sr320 commented 1 year ago

I did locate four more bags

Thus to date it is likely

Orange = ? Pink = ? Blue = ? Yellow = F14 (2n) Dog [Putnam hobo] = F5 (2n)? Cat [very big, few] = F5 (3n) Bird [Tank 3 hobo] = F13 (either 2n or 3n) Toad = F14 (3n)

AHuffmyer commented 5 months ago

Do we have updates to this list or do unknowns remain unknown?

sr320 commented 5 months ago

No, we really need to coordinate with Pacific hybrid to determinative if they can id with cytometer (dip vs trip)

On Thu, Apr 4, 2024 at 11:15 AM Ariana S Huffmyer @.***> wrote:

Do we have updates to this list or do unknowns remain unknown?

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AHuffmyer commented 5 months ago

Is that something we can do or would we need to send them animals?

mattgeorgephd commented 5 months ago

Benoit at Quilcene Hatchery also has a ploidy analyzer

sr320 commented 5 months ago

Alexa (PH) has agreed to do this. The next step is for me to somehow get the oysters to her in Manchester.

AHuffmyer commented 5 months ago

I can take oysters to Manchester next week - I could pick up on a Point Whitney day and deliver to Manchester.

AHuffmyer commented 4 months ago

Delivered to Manchester on 5/7; Alexa analyzing on 4/8

AHuffmyer commented 3 months ago

Results from analyzed cohorts here: https://ahuffmyer.github.io/ASH_Putnam_Lab_Notebook/Oyster-ploidy-results/

Need to confirm other families not detailed in this post

sr320 commented 3 months ago

before

Orange = ? Pink = ? Blue = ? Yellow = F14 (2n) Dog [Putnam hobo] = F5 (2n)? Cat [very big, few] = F5 (3n) Bird [Tank 3 hobo] = F13 (either 2n or 3n) Toad = F14 (3n)

AFTER analyzes

Orange = ? triploid Pink = ? Blue = ? Tetraploid Yellow = F14 (2n) diploid Dog [Putnam hobo] = F5 (2n)? triploid Cat [very big, few] = F5 (3n) Bird [Tank 3 hobo] = F13 (either 2n or 3n) triploid Toad = F14 (3n)

AHuffmyer commented 6 days ago

I believe we have done all the identification we can do up to this point, correct? I'll close if so.