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Extract DNA and RNA from 6 cod blood samples #1951

Open sr320 opened 1 month ago

laurahspencer commented 1 month ago

Cod blood samples for this test DNA + RNA extraction. I've provided 8 (4 each for treatment, 5C and 9C) in case any of these samples are missing or have very little blood.

Temperature Sample #
5 129
5 149
5 154
5 155
9 84
9 93
9 111
9 114
kubu4 commented 6 days ago

Alrighty, this is what we're looking at.

Here's notebook entry:

https://robertslab.github.io/sams-notebook/posts/2024/2024-08-29-DNA-and-RNA-Isolations---Gadus-macrocephalus-Blood/

Elution volumes were 15uL for DNA and 15uL for RNA.

Sample_ID Concentration(ng/uL)
84 12.5
93 3.42
111 18.3
114 58.8
129 0
149 20.8
154 7.76
Sample_ID Concentration(ng/uL)
84 18.6
93 28
111 22.8
114 20.6
129 7.9
149 22.2
154 21.8

I couldn't locate sample 155, BTW.

I think isolations could've been better. The blood coagulated into a dense goo as soon as I added the lysis buffer/proteinase k and didn't ever really break down. I probably should've tried to physically homogenize the samples after they lysis step showed minimal impact...