May I ask how to add the acetylated N-terminus and methylamidated C-terminus to a pose when using Pyrosetta? The pose is loaded from a pdb using a command like:
pose = pose_from_pdb("test.pdb").
I googled as much as I possible, but still cann't find a command that can work.
There was a command seemed to worked, but still it didn't:
pyrosetta.rosetta.core.conformation.add_variant_type_to_conformation_residue(pose.conformation(), pyrosetta.rosetta.core.chemical.ACETYLATED_NTERMINUS_VARIANT, 1)
The error was:
ERROR: Unable to find desired residue 'LYS:NtermProteinFull' with variant 'ACETYLATED_NTERMINUS_VARIANT'. Attempted to add target variant(s) to ResidueType using both ResidueType base name 'LYS' and base ResidueType. Was attempting to add new variant type 'ACETYLATED_NTERMINUS_VARIANT'
Hi team,
May I ask how to add the acetylated N-terminus and methylamidated C-terminus to a pose when using Pyrosetta? The pose is loaded from a pdb using a command like: pose = pose_from_pdb("test.pdb").
I googled as much as I possible, but still cann't find a command that can work. There was a command seemed to worked, but still it didn't: pyrosetta.rosetta.core.conformation.add_variant_type_to_conformation_residue(pose.conformation(), pyrosetta.rosetta.core.chemical.ACETYLATED_NTERMINUS_VARIANT, 1)
The error was: ERROR: Unable to find desired residue 'LYS:NtermProteinFull' with variant 'ACETYLATED_NTERMINUS_VARIANT'. Attempted to add target variant(s) to ResidueType using both ResidueType base name 'LYS' and base ResidueType. Was attempting to add new variant type 'ACETYLATED_NTERMINUS_VARIANT'
Thank you very much.
All the best.