Closed wfgui closed 2 years ago
I use the the software to construct the CRISPRCas database from the bacterial genome in the Refseq database. But I found that some spacer sequences are not in the genome,for example(spacers/NC_015711.1_1.fa):>NC_015711.1_1:1
>NC_015711.1_1:1
>NC_015711.1_1:1 TCAACCAGCATTAGCACCGTCCGCGTGGCGCCCGTGT >NC_015711.1_1:2 CTGGAGTTGTCCCCCGAGGCTGAGCCGGTGTCCCGCGT >NC_015711.1_1:3 TCTTCCATCTGCGTCTGCGTCTGACCCTTGAACTTCG >NC_015711.1_1:4 ATGCAGAACAGCGGCAAGGAGGCGATTATCGACCT >NC_015711.1_1:5 GGGCAGTGAAACCCTTGGGTGGGGAAGGAGTTCTGGGGGC >NC_015711.1_1:6 AGGAGCGCCCGCCGGCCAGACGCATAGACGACGCA
Am i missing something?
Thanks!
GCF_000219105.zip
Dear wfgui,
Not sure what the problem is here. When I blast these 6 spacer sequences against NC_015711.1 I find perfect matches for all, as expected.
See attached BLASTn table: BLASTn-Alignment.txt
Cheers, Jakob
I use the the software to construct the CRISPRCas database from the bacterial genome in the Refseq database. But I found that some spacer sequences are not in the genome,for example(spacers/NC_015711.1_1.fa):
>NC_015711.1_1:1
Am i missing something?
Thanks!
GCF_000219105.zip