Russel88 / CRISPRCasTyper

CCTyper: Automatic detection and subtyping of CRISPR-Cas operons
https://typer.crispr.dk
MIT License
89 stars 16 forks source link

Complete genome and draft genome #31

Closed illusion-a closed 1 year ago

illusion-a commented 1 year ago

Hello, I am going to use this software to predict some Klebsiella pneumoniae genome data (some are fully assembled genome data, some are draft genome data at the Scaffolds level). Should I change the Prodigal Mode to make the prediction more accurate for different genomes? Complete genome: cctyper KP1.fa my_output A draft genome containing multiple sequences(scaffolds): cctyper KP2.fasta my_output2 --prodigal meta Are these commands correct? If I keep using a command without changing it, will the result be much less reliable ? Best wishes!

Russel88 commented 1 year ago

Hi The default mode is best for when the input fasta contains a single genome, no matter if it's complete or draft. Prodigal meta mode is only for short sequences or fasta files with contigs from different bacteria (e.g. metagenomes). So it sounds like you should just use default mode: cctyper genome.fa my_output. Cheers, Jakob

illusion-a commented 1 year ago

I see. Thank you very much!