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SBC-Utrecht / PyTom

PyTom software for cryo-tomogram processing
GNU General Public License v2.0
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3D template matching in VPP_tomo #61

Open yanhezhao opened 1 month ago

yanhezhao commented 1 month ago

Greeting there,

I am new to the PyTom. I have several datasets were collected with VPP and defocus 0.5um. I want to confirm before I start that whether the 3D template matching will works on this type datasets?

Thanks a lot

sroet commented 1 month ago

Hey @yanhezhao,

If you just want template matching, I would advise you to use our improved template matching code instead: https://github.com/SBC-Utrecht/pytom-match-pick

In there you can definitely supply the --defocus and --phase-shift!

alncat commented 1 month ago

@yanhezhao It definitely works. You only need to confirm that if particle is white, background is dark, then you reference should also be white, vice versa.