SCA-IRCM / SingleCellSignalR

R package for Bioconductor submission
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NA output in all cell_signalling tables #16

Closed jhb1980 closed 3 years ago

jhb1980 commented 3 years ago

Hi! I'm trying to run the cell_signaling function on data generated with Seurat. Verbose output suggests it successfully finds several interactions across different clusters. In the output, all fields including row names however are filled with "NA" values. The function generates warnings, these are captured as below.

50: In graph_from_data_frame(final, directed = FALSE) :
  In `d' `NA' elements were replaced with string "NA"

Function call looks like:

signal <- cell_signaling(data = data.sub,
  genes = test.genes,
  cluster = as.numeric(Idents(s.sub)),
  c.names = levels(s.sub),
  s.score = 0.5,
  logFC = log2(1.5),
  write = FALSE)
jhb1980 commented 3 years ago

Solved by forcing a resintall of dependencies in the conda environment.