Closed GoogleCodeExporter closed 8 years ago
Processing chr1
Initializing to length 249250621
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr1.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 3.73992e-06
Refining 211 boundaries
Loading reads from input file /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3-Duplicate-236-8.hits.........
Importing bowtie formatted reads
Skipped 0 reads
Imported 4811071 inpput reads
Sorting reads ...COMPLETE
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr1_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr1_win250bp_offset125bp.txt
Processing chr2
Initializing to length 243199373
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr2.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.3066e-06
Refining 230 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr2_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr2_win250bp_offset125bp.txt
Processing chr3
Initializing to length 198022430
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr3.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.23328e-06
Refining 201 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr3_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr3_win250bp_offset125bp.txt
Processing chr4
Initializing to length 191154276
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr4.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 1.40515e-06
Refining 179 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr4_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr4_win250bp_offset125bp.txt
Processing chr5
Initializing to length 180915260
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr5.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.67944e-06
Refining 141 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr5_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr5_win250bp_offset125bp.txt
Processing chr6
Initializing to length 171115067
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr6.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 3.34593e-06
Refining 130 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr6_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr6_win250bp_offset125bp.txt
Processing chr7
Initializing to length 159138663
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr7.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.36365e-06
Refining 124 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr7_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr7_win250bp_offset125bp.txt
Processing chrX
Initializing to length 155270560
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chrX.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 6.45025e-07
Refining 127 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chrX_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chrX_win250bp_offset125bp.txt
Processing chr8
Initializing to length 146364022
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr8.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 1.60753e-06
Refining 133 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr8_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr8_win250bp_offset125bp.txt
Processing chr9
Initializing to length 141213431
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr9.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.32899e-06
Refining 114 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr9_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr9_win250bp_offset125bp.txt
Processing chr10
Initializing to length 135534747
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr10.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.12094e-06
Refining 134 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr10_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr10_win250bp_offset125bp.txt
Processing chr11
Initializing to length 135006516
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr11.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 4.2534e-06
Refining 95 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr11_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr11_win250bp_offset125bp.txt
Processing chr12
Initializing to length 133851895
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr12.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 3.27651e-06
Refining 112 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr12_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr12_win250bp_offset125bp.txt
Processing chr13
Initializing to length 115169878
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr13.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 1.44439e-06
Refining 90 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr13_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr13_win250bp_offset125bp.txt
Processing chr14
Initializing to length 107349540
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr14.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 3.28852e-06
Refining 78 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr14_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr14_win250bp_offset125bp.txt
Processing chr15
Initializing to length 102531392
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr15.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.67231e-06
Refining 94 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr15_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr15_win250bp_offset125bp.txt
Processing chr16
Initializing to length 90354753
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr16.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 4.05903e-06
Refining 65 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr16_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr16_win250bp_offset125bp.txt
Processing chr17
Initializing to length 81195210
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr17.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 5.21264e-06
Refining 68 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr17_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr17_win250bp_offset125bp.txt
Processing chr18
Initializing to length 78077248
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr18.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 1.31377e-06
Refining 80 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr18_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr18_win250bp_offset125bp.txt
Processing chr20
Initializing to length 63025520
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr20.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.36898e-06
Refining 73 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr20_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr20_win250bp_offset125bp.txt
Processing chrY
Initializing to length 59373566
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chrY.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 2.68433e-07
Refining 19 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chrY_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chrY_win250bp_offset125bp.txt
Processing chr19
Initializing to length 59128983
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr19.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 6.56945e-06
Refining 68 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr19_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr19_win250bp_offset125bp.txt
Processing chr22
Initializing to length 51304566
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr22.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 3.49813e-06
Refining 43 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr22_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr22_win250bp_offset125bp.txt
Processing chr21
Initializing to length 48129895
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chr21.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 1.98445e-06
Refining 34 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr21_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chr21_win250bp_offset125bp.txt
Processing chrM
Initializing to length 16571
Mapping reads to chromosome......
Getting alignability info from:
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_200/chrM.wig
Getting sequence from .2bit file:
/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit
Getting counts for 100000bp windows..........
Refining boundaries....
Global variance is 1.68319e-06
Refining 0 boundaries
Mapping input tags to the genome.........
Getting counts for zinba windows..........
Offset 0bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chrM_win250bp_offset0bp.txt
Offset 125bp......
Printed out data to /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236-8_chrM_win250bp_offset125bp.txt
--------BUILD WINDOWS COMPLETE-------
Original comment by por...@porter.st
on 3 Feb 2012 at 3:26
--------STARTING MODEL SELECTION-------- 2012-02-02 18:13:10
$`1`
exp_count ~ 1
<environment: 0x399c688>
$`2`
exp_count ~ input_count + 1
<environment: 0x399c688>
...............................[1] "The estimated proportion of enrichment for
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr22_win250bp_offset0bp.txt has exceeded 0.5, suggesting difficulty in
estimating enrichment. Switching to more conservative model"
model 3 completed out of 8 ............[1] "The estimated proportion of
enrichment for
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr22_win250bp_offset0bp.txt has exceeded 0.5, suggesting difficulty in
estimating enrichment. Switching to more conservative model"
model 4 completed out of 8 ...........................
model 2 completed out of 8
...............................................................................
................................................................................
..............................................
model 6 completed out of 8 ........................
model 1 completed out of 8
...............................................................................
................................................................................
.............................................
model 5 completed out of 8 ..
model 8 completed out of 8
...............................................................................
......................................................
model 7 completed out of 8 Background formula is:
exp_count ~ input_count + 1
<environment: 0x399c688>
Enrichment formula is:
exp_count ~ 1
<environment: 0x399c688>
Zero-inflated formula is:
exp_count ~ 1
<environment: 0x399c688>
--------MODEL SELECTION COMPLETE-------- 2012-02-02 18:56:51
--------GETTING ENRICHED WINDOWS-------- 2012-02-02 18:56:51
.......................................................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr4_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 19:44:02 ..
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr3_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 19:45:26 ...
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr1_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 19:48:09 .......
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr2_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 19:53:43 .....................................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr4_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 20:22:28
Time difference of 1.430973 hours
...
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr3_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 20:25:09
Time difference of 1.476377 hours
.........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr1_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 20:31:53
Time difference of 1.588956 hours
.........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr2_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 20:38:29
Time difference of 1.69926 hours
......................................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr6_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 21:02:13 ...
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr5_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 21:04:02 .......
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr7_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 21:07:24 ..........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chrX_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 21:14:10 ..................................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr6_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 21:34:42
Time difference of 1.158196 hours
..
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr7_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 21:36:45 .
Time difference of 1.079499 hours
........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr5_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 21:42:07
Time difference of 1.327453 hours
.............
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chrX_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 21:49:12 .
Time difference of 1.176672 hours
............................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr9_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 22:03:02 ..........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr8_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 22:07:47 .......
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr10_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 22:11:52 ...............
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr11_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 22:18:26 ................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr9_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 22:26:44
Time difference of 49.93117 mins
.................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr8_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 22:34:32
Time difference of 59.79024 mins
........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr10_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 22:38:30
Time difference of 56.30224 mins
..............
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr11_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 22:44:17
Time difference of 55.04764 mins
...............................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr12_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 22:54:45 ..........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr13_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 22:58:31 ...
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr14_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 22:59:52 ..............
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr15_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 23:04:05 ...................................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr13_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 23:17:07 .
Time difference of 42.52728 mins
..
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr14_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 23:17:48
Time difference of 39.25314 mins
....
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr12_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 23:19:29
Time difference of 52.77208 mins
....
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr15_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 23:21:01
Time difference of 36.67555 mins
........................................................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr20_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 23:34:44 .....
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr17_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 23:36:10 ..
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr16_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 23:36:34 .....
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr18_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 23:37:57 ............................................................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chrY_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-02 23:49:41 ..........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr17_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 23:51:33
Time difference of 33.73838 mins
.....
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr16_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 23:52:40 .
Time difference of 35.524 mins
..
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr18_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 23:53:03 .
Time difference of 33.47804 mins
............................................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chrY_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-02 23:58:47 .
Time difference of 18.30639 mins
....................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr22_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-03 00:01:34 .
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr21_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-03 00:01:40 ...................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr19_win250bp_offset0bp.txt
Selected number of peaks: 0
2012-02-03 00:04:58 ...................
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr22_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-03 00:08:27
Time difference of 15.72667 mins
....
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr21_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-03 00:09:16
Time difference of 16.16309 mins
........
Processed
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3-Duplicate-236
-8_chr19_win250bp_offset125bp.txt
Selected number of peaks: 0
2012-02-03 00:14:43
Time difference of 23.12095 mins
--------WINDOW ANALYSIS COMPLETE-------- 2012-02-03 00:14:47
--------MERGE WINDOWS AND REFINE PEAKS (no parallelization)-------- 2012-02-03
00:14:47
Threshold is 0.05
Getting significant windows from
/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3.winlist
Threshold is 0.050000
Using qvalue as threshold
Data generated using mixture
Data format is expanded
Distance to collapse windows is 0
Getting significant regions from mixture run
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr1.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr2.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr3.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr4.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr5.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr6.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr7.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chrX.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr8.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr9.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr10.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr11.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr12.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr13.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr14.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr15.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr16.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr17.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr18.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chrY.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr19.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr22.wins...
Importing windows from /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr21.wins...
Skipping line, /mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3_files/Naive-H3K4me3_chr21.wins
Imported 0 coordinates
*** glibc detected *** /usr/lib64/R/bin/exec/R: double free or corruption
(out): 0x00007fff32499cb0 ***
======= Backtrace: =========
/lib64/libc.so.6(+0x74c06)[0x7f004fd73c06]
/usr/lib64/R/library/zinba/libs/zinba.so(+0x3af4a)[0x7f004c6c2f4a]
/usr/lib64/R/library/zinba/libs/zinba.so(_ZN8analysis12importCoordsEPKcdS1_iii+0
x18f)[0x7f004c6c480f]
/usr/lib64/R/library/zinba/libs/zinba.so(getSeqCountProfile+0x109)[0x7f004c710fa
9]
/usr/lib64/R/lib/libR.so(+0xb2f97)[0x7f005035df97]
/usr/lib64/R/lib/libR.so(Rf_eval+0x739)[0x7f005038e129]
/usr/lib64/R/lib/libR.so(+0xe5da0)[0x7f0050390da0]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(+0xe5f29)[0x7f0050390f29]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_applyClosure+0x322)[0x7f0050392142]
/usr/lib64/R/lib/libR.so(Rf_eval+0x430)[0x7f005038de20]
/usr/lib64/R/lib/libR.so(+0xe5f29)[0x7f0050390f29]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_applyClosure+0x322)[0x7f0050392142]
/usr/lib64/R/lib/libR.so(Rf_eval+0x430)[0x7f005038de20]
/usr/lib64/R/lib/libR.so(+0xe5f29)[0x7f0050390f29]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(+0xe5f29)[0x7f0050390f29]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(+0xe5f29)[0x7f0050390f29]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_applyClosure+0x322)[0x7f0050392142]
/usr/lib64/R/lib/libR.so(Rf_eval+0x430)[0x7f005038de20]
/usr/lib64/R/lib/libR.so(+0xe5f29)[0x7f0050390f29]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(+0xe5f29)[0x7f0050390f29]
/usr/lib64/R/lib/libR.so(Rf_eval+0x55b)[0x7f005038df4b]
/usr/lib64/R/lib/libR.so(Rf_applyClosure+0x322)[0x7f0050392142]
/usr/lib64/R/lib/libR.so(Rf_eval+0x430)[0x7f005038de20]
/usr/lib64/R/lib/libR.so(+0xe425d)[0x7f005038f25d]
/usr/lib64/R/lib/libR.so(+0xd7d23)[0x7f0050382d23]
/usr/lib64/R/lib/libR.so(Rf_eval+0x17a)[0x7f005038db6a]
/usr/lib64/R/lib/libR.so(Rf_applyClosure+0x322)[0x7f0050392142]
/usr/lib64/R/lib/libR.so(+0xded84)[0x7f0050389d84]
/usr/lib64/R/lib/libR.so(Rf_eval+0x17a)[0x7f005038db6a]
/usr/lib64/R/lib/libR.so(Rf_applyClosure+0x322)[0x7f0050392142]
/usr/lib64/R/lib/libR.so(Rf_eval+0x430)[0x7f005038de20]
/usr/lib64/R/lib/libR.so(Rf_ReplIteration+0x1e3)[0x7f00503c34d3]
/usr/lib64/R/lib/libR.so(+0x118760)[0x7f00503c3760]
/usr/lib64/R/lib/libR.so(run_Rmainloop+0x50)[0x7f00503c3c80]
/usr/lib64/R/bin/exec/R(main+0x1b)[0x4007fb]
/lib64/libc.so.6(__libc_start_main+0xed)[0x7f004fd2023d]
/usr/lib64/R/bin/exec/R[0x40082d]
======= Memory map: ========
00400000-00401000 r-xp 00000000 08:01 1462739
/usr/lib64/R/bin/exec/R
00600000-00601000 r--p 00000000 08:01 1462739
/usr/lib64/R/bin/exec/R
00601000-00602000 rw-p 00001000 08:01 1462739
/usr/lib64/R/bin/exec/R
00602000-05b4f000 rw-p 00000000 00:00 0 [heap]
7f004bf74000-7f004bf79000 r-xp 00000000 08:01 272620
/usr/lib64/R/library/MASS/libs/MASS.so
7f004bf79000-7f004c178000 ---p 00005000 08:01 272620
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[vsyscall]
Aborted
Original comment by por...@porter.st
on 3 Feb 2012 at 3:27
> R.version
_
platform x86_64-suse-linux-gnu
arch x86_64
os linux-gnu
system x86_64, linux-gnu
status
major 2
minor 14.1
year 2011
month 12
day 22
svn rev 57956
language R
version.string R version 2.14.1 (2011-12-22)
> library(zinba)
version of zinba is 2.01
Original comment by por...@porter.st
on 3 Feb 2012 at 3:28
From what it looks like ZINBA could not detect any peaks regions with
confidence in your data set, so did not create any output in the enrichment
detection step (leading to a problem in the later step). It appears your read
depth seems to be too low to detect peaks with the default parameters. You can
try using the run.zinba function (detailed in the Using Zinba wiki) and use a
larger window size and see if that improves the results. If you want to get a
better picture of what your data looks like, you can load your .basecount file
(it is in .wig format) into a locally installed version of the UCSC genome
browser.
I am working on an update coming out this weekend that will prevent the above
segfault from occurring when no peaks are selected. It may also be useful for
ZINBA to print out some chromosome-level window read count summary information
to provide an idea of what the level of signal that ZINBA is working with.
Original comment by homer...@gmail.com
on 3 Feb 2012 at 4:02
Sounds good. Also I found that I've used mappability 36bp when data is 25bp, is
it a big problem, should I recreate my own mappability file for 25bp reads?
Original comment by por...@porter.st
on 3 Feb 2012 at 4:19
One more option is to use the run.zinba function with the default window size
(250bp) and modelselect=F, specifying your own formulas. For example, the
formula selected in your output is the following
background: exp_count ~ input_count + 1
enrichment: exp_count ~ 1
zero-inflation: exp_count ~ 1
With just input as a covariate, I've only seen these formulas chosen in cases
where ZINBA cannot detect any signal that differs above background. Using the
following formulas in run.zinba may alleviate the low coverage situation, but
no guarantee:
modelselect=F,
formula= exp_count ~ input_count + 1,
formulaE= exp_count ~ input_count + 1,
formulaZ= exp_count ~ 1,
Original comment by homer...@gmail.com
on 3 Feb 2012 at 4:20
I dont think it will make much of a difference 25 vs 36, so you should be ok
Original comment by homer...@gmail.com
on 3 Feb 2012 at 4:27
Also, in some cases modeling your data without input control (leaving
input='none' in zinba() function ) may be more beneficial in low depth cases
than using input control, so that may be a better option. If this works better
for you let me know.
Original comment by homer...@gmail.com
on 3 Feb 2012 at 4:29
I'll try all these variations and report back a result.
Thanks
Original comment by por...@porter.st
on 3 Feb 2012 at 4:35
I'm really sorry, I used your parameters and received this error
source("./ZINBA/com.R")
Error in zinba(align = "/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_150/", :
unused argument(s) (winSize = 250, modelselect = F, formula = exp_count ~ input_count + 1, formulaE = exp_count ~ input_count + 1, formulaZ = exp_count ~ 1)
command is:
zinba(
align='/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/output_150/',
numProc=4,
seq='/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3-Duplicate-236-8.hits',
basecountfile='/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3-Duplicate-236-8.basecount',
filetype='bowtie',
outfile='/mnt/sf_G_DRIVE/FASTQ-DATA/ZINBA/Naive-H3K4me3',
twoBit='/mnt/sf_G_DRIVE/FASTQ-DATA/b_indexes/hg19.2bit',
extension=150,
printFullOut=1,
refinepeaks=1,
broad=F,
winSize=250,
modelselect=F,
formula= exp_count ~ input_count + 1,
formulaE= exp_count ~ input_count + 1,
formulaZ= exp_count ~ 1
)
Original comment by por...@porter.st
on 3 Feb 2012 at 5:44
If you look at the Using ZINBA tutorial, the zinba() function does not use
these functions. To specify them, you have to use the *run.zinba* function, as
mentioned earlier.
Original comment by homer...@gmail.com
on 3 Feb 2012 at 5:58
I've attached two error files with different winSize parameter, R still crushed.
Original comment by por...@porter.st
on 3 Feb 2012 at 7:43
Attachments:
Please follow the run.zinba example here on the Using Zinba page here, using
the *CHiP-seq example* as a template for how to run the function:
http://code.google.com/p/zinba/wiki/UsingZINBA#Custom_Analysis . You are
ignoring several parameters, leading to the crashes. The new version coming
this weekend will be more stable with this function.
Original comment by homer...@gmail.com
on 3 Feb 2012 at 8:16
I've made as you said, and it done looks like well. I did not test 2.02, I'll
do it on next week. Also I almost add .bam file loading (like "bowtie" type)
are you interested in that ?
Zinba done well on histone precipitation and not so understandable on DNase.
Maybe on DNase data, I should play with some sensitivity parameters, because
near highly enreached regions it found some times peaks where they look like
background. I'm not a big professional, so maybe I'm wrong somewhere.
Original comment by por...@porter.st
on 12 Feb 2012 at 4:54
Here are some suggested parameters by one of our users that applied zinba to
DNAse data. In general the results seemed ok to them, except with the FDR=T
option there was some overcalling of peak (may need a more strict threshold).
Feel free to contact me directly at homeriq5@gmail.com with any suggests or
comments.
zinba(
align='[% alignability_directory %]',
numProc=[% number_of_processors %],
seq='[% seq_file %]',
basecountfile='[% basecount_file %]',
filetype='[% seq_file_type %]',
outfile='[% output_dir_prefix %]',
threshold=.05,
twoBit='[% two_bit_file %]',
extension=1,
refinepeaks=1,
printFullOut=1,
broad=F,
input="none"
)
Original comment by homer...@gmail.com
on 16 Feb 2012 at 7:39
Original comment by homer...@gmail.com
on 16 Feb 2012 at 7:39
Original issue reported on code.google.com by
por...@porter.st
on 3 Feb 2012 at 3:26