Closed DianaSpurite closed 2 years ago
Note you've pasted the sample data into the library column
Adding Guellil 2021 samples. Here I would like to ask for double checking of the genome _type as the paper also reports plasmids;
In terms of genome_type, we report whatever is the largest available, chromosome > organelle
data_type and also sample_name as some of the sample names repeat.
in this case you should only jave one sample_name
- IMO2, as this is the only one they actually generated a genome out of (via capture)
Following the screening of shotgun metagenomic datasets, we enriched libraries of sample IMO2 for the plague pathogen Y. pestis, using a custom RNA baits design.
Shotgun sequencing (screening) is to see if there are any DNA from that species in the sample, then for single-genome pathogen studies they normally will enrich via bait capture to get the whole genome
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
run accession SRR13608699, so in SRA, must correct that
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json
Errors were found, please unfold below to see errors:
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
Seems it has finally worked. Please review, when you get a moment.
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
Samples look good , but you have not removed the non-IMO2 samples from the library table! Check the
sample_title
column of the ENA table for guidanceOnly include IMO02{a,b,c,d...} libraries in the list!
To me it appears that I have only the IMO2's (IMO2a lib I.I2; IMO2a lib. IC7; IMO2b lib. IC11; IMO2a lib. IC14; IMO2d lib. IC15; IMO2e lib. IC18; IMO2f lib. IC19). Which ones, should I remove?
Samples look good , but you have not removed the non-IMO2 samples from the library table! Check the
sample_title
column of the ENA table for guidance Only include IMO02{a,b,c,d...} libraries in the list!To me it appears that I have only the IMO2's (IMO2a lib I.I2; IMO2a lib. IC7; IMO2b lib. IC11; IMO2a lib. IC14; IMO2d lib. IC15; IMO2e lib. IC18; IMO2f lib. IC19). Which ones, should I remove?
Oops you're right, I misread sorry (eyes were getting crossed...), you've got everything OK in terms of correct librareis :+1:
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
ancientMetagenomeDirCheck, version 1.3
Checking ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s.tsv against schema ancientmetagenome-hostassociated/samples/ancientmetagenome-hostassociated_sample s_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples.tsv against schema ancientsinglegenome-hostassociated/samples/ancientsinglegenome-hostassociated_sa mples_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/samples/ancientmetagenome-environmental_samples. tsv against schema ancientmetagenome-environmental/samples/ancientmetagenome-environmentalsamples schema.json All is good, no errors were found !
Checking ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries.tsv against schema ancientmetagenome-hostassociated/libraries/ancientmetagenome-hostassociated_libr aries_schema.json All is good, no errors were found !
Checking ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries.tsv against schema ancientsinglegenome-hostassociated/libraries/ancientsinglegenome-hostassociated libraries_schema.json All is good, no errors were found !
Checking ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies.tsv against schema ancientmetagenome-environmental/libraries/ancientmetagenome-environmental_librar ies_schema.json All is good, no errors were found !
Thakns @DianaSpurite !
Adding Guellil 2021 samples. Here I would like to ask for double checking of the genome _type as the paper also reports plasmids; data_type and also sample_name as some of the sample names repeat.
Pull Request
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This pull request is to add samples from the following publication(s): https://www.nature.com/articles/s41598-021-98214-2
This is to close #736
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This pull request is to correct samples from the following publication(s):
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