Closed sarahshuang closed 2 months ago
Hi, it is possible that your network has restrictions on accessing Docker hub.
Hi, it is possible that your network has restrictions on accessing Docker hub.
I see. Does it have other approaches to build SAW successfully?
Hi, it is possible that your network has restrictions on accessing Docker hub.
I see. Does it have other approaches to build SAW successfully?
Hi, could I ask if you need to analyze proteomics data? If you only need to analyze Stereo-seq transcriptome data, we recommend that you could use the latest SAW v8, which only need to be unzipped to use.
https://www.stomics.tech/products/BioinfoTools/OfflineSoftware https://en.stomics.tech/products/BioinfoTools/OfflineSoftware
Hi, it is possible that your network has restrictions on accessing Docker hub.
I see. Does it have other approaches to build SAW successfully?
Hi, could I ask if you need to analyze proteomics data? If you only need to analyze Stereo-seq transcriptome data, we recommend that you could use the latest SAW v8, which only need to be unzipped to use.
https://www.stomics.tech/products/BioinfoTools/OfflineSoftware https://en.stomics.tech/products/BioinfoTools/OfflineSoftware
I only analyze transcriptome data. Great! I will try. Thank you!
Hi, it is possible that your network has restrictions on accessing Docker hub.
I see. Does it have other approaches to build SAW successfully?
Hi, could I ask if you need to analyze proteomics data? If you only need to analyze Stereo-seq transcriptome data, we recommend that you could use the latest SAW v8, which only need to be unzipped to use.
https://www.stomics.tech/products/BioinfoTools/OfflineSoftware https://en.stomics.tech/products/BioinfoTools/OfflineSoftware
Hi,
I have a new question. I am running SAW v8 with your public demo data [C04138G3_v8.0_mouse_brain_Stereo seq_N_FFPE], but I only get saw count
. Why doesn't it have <.cellbin.gef> as well?
@sarahshuang Yes, in the current v8.0 version, automatic cell segmentation will not be performed for H&E stained images of FFPE samples. In this case, it is recommended that users use third-party tools for cell segmentation and then import the result into SAW for cellbin analysis.
@sarahshuang Yes, in the current v8.0 version, automatic cell segmentation will not be performed for H&E stained images of FFPE samples. In this case, it is recommended that users use third-party tools for cell segmentation and then import the result into SAW for cellbin analysis.
@sarahshuang Yes, in the current v8.0 version, automatic cell segmentation will not be performed for H&E stained images of FFPE samples. In this case, it is recommended that users use third-party tools for cell segmentation and then import the result into SAW for cellbin analysis.
Got it. Thank you!
Hi,
I am encounted with the following connection error. Do you have some suggestions?