STOmics / Stereopy

A toolkit of spatial transcriptomic analysis.
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cell segmentation tutorial fail to generate the results. #137

Closed limin321 closed 1 year ago

limin321 commented 1 year ago

Hi, I followed cell segmentation tutorial to set up running V3, V1, and DeepCell model. Based on the scripts, the 3 models all ran with no issue. However, the image results are different from the tutorial.

Here is the output of V1 model:

Screen Shot 2023-06-27 at 3 21 45 PM

Here is the output of

Screen Shot 2023-06-27 at 3 22 12 PM

However, V3 model only has one file called SS200000135TL_D1_regist.cell_cut.tif. First question, how many files do I expect to see from V3 model?

Second question, when I open the XX_regist_mask.tif, here is what I see. V1 model mask.tif

Screen Shot 2023-06-27 at 3 28 32 PM

DeepCell model mask.tif

Screen Shot 2023-06-27 at 3 29 19 PM

V3 model doesn't have this mask.tif file.

Really appreciate your help,

Best,

LC

TheSallyGardens commented 1 year ago

@limin321 Hi! Question 1 : V3 model has only one output, namely SS200000135TL_D1_regist.cell_cut.tif. Question 2 : The tif generated by the DeepCell model can be viewed by adjusting the contrast using imageJ.

Best,

TheSallyGardens

limin321 commented 1 year ago

@limin321 Hi! Question 1 : V3 model has only one output, namely SS200000135TL_D1_regist.cell_cut.tif. Question 2 : The tif generated by the DeepCell model can be viewed by adjusting the contrast using imageJ.

Best,

TheSallyGardens

@TheSallyGardens ,

Thank you so much for the help. It works in imageJ, though my images of the 3 models are not colorful. I am also able to generate the cell-bin files for them. I am going to close my this topic.

Best, LC