mkdoherty
commented
8 years ago Bacterial 16S rRNA pyrosequencing data sets have been deposited in EMBL European Bioinformatics Institute (EBI) under the accession numbers ERP005633, ERP005634, ERP005636, and ERP005637. Shotgun pyrose- quencing data sets of community DNA are available in EBI under the accession number ERP005635. GeneChip data have been deposited in Gene Expression Omnibus (GSE57589).
Seedorf, H. Griffin, N. W. Ridaura, V. K. Reyes, A. Cheng, J. Rey, F. E. Smith, M. I. Simon, G. M. Scheffrahn, R. H. Woebken, D. Spormann, A. M. Van Treuren, W. Ursell, L. K. Pirrung, M. Robbins-Pianka, A. Cantarel, B. L. Lombard, V. Henrissat, B. Knight, R. Gordon, J. I. 2014 Bacteria from diverse habitats colonize and compete in the mouse gut http://dx.doi.org/10.1016/j.cell.2014.09.008
To study how microbes establish themselves in a mammalian gut environment, we colonized germ-free mice with microbial communities from human, zebrafish, and termite guts, human skin and tongue, soil, and estuarine microbial mats. Bacteria from these foreign environments colonized and persisted in the mouse gut; their capacity to metabolize dietary and host carbohydrates and bile acids correlated with colonization success. Cohousing mice harboring these xenomicrobiota or a mouse cecal microbiota, along with germ-free "bystanders," revealed the success of particular bacterial taxa in invading guts with established communities and empty gut habitats. Unanticipated patterns of ecological succession were observed; for example, a soil-derived bacterium dominated even in the presence of bacteria from other gut communities (zebrafish and termite), and human-derived bacteria colonized germ-free bystander mice before mouse-derived organisms. This approach can be generalized to address a variety of mechanistic questions about succession, including succession in the context of microbiota-directed therapeutics.