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NGS (genomic DNA) analyses using Galaxy #12

Closed Tsaaki1 closed 6 years ago

Tsaaki1 commented 6 years ago

Hi,

I am using Galaxy to analyze my sequencing data. Ultimately I want to see SNPs in the sequenced genome. I was able to generate mapping on the reference genome in the BAM format. But I am stuck here. The next thing would be Variant calling. When I perform this step, I cannot properly view these SNPs. Does anyone have a workflow for this kind of analyses and willing to share?

My workflow is essentially:

  1. Get Data
  2. FastQC on 1
  3. MultiQC 2
  4. NGS: QC and manipulation, Trimmomatic on 1
  5. FastQC on 4
  6. MultiQC on 5
  7. Mapping: Bowtie2 on 4
mgalland commented 6 years ago

Hello Terence, Seems like you're the first to post a real issue here (and not a lesson). We sure can help. If you're willing to wait a little bit (10 days), we will plan so-called "hacky hour" where you can come to solve issues like this one. In the past, we had an irregular number of people joining these "hacky hours". Thus, now, what I'm planning to do is to ask at workshop sessions (next one on November 21) how many people would like to join the following week hacky hour. Then, we'll know how many people actually come. I don't have Galaxy workflows (I'm interested though to see how you use it) but rather I have workflows made with Snakemake. Please, come to the next Study Group sessions (November 21th) as we will see how many people Best, Marc Ps: did you search on Biostars or Seqanswers forums?

Tsaaki1 commented 6 years ago

@mgalland thank you for comments. I will definitely come to the next session. In the meantime, I will go through your suggestions. I have not go through Biostars and SeqAnwers.

regards, Terrens

mgalland commented 6 years ago

Hey Terence, Any update on this?