I have assembly sequences for over 300 different pathogens, and I want to use multiplex PCR to determine if a sample contains a particular pathogen. However, since I am targeting the entire genome rather than specific regions, I would like to know if your tool is suitable for designing multiplex primers under these circumstances.
Thank you for your assistance. I look forward to your guidance.
Dear @SemiQuant
I have assembly sequences for over 300 different pathogens, and I want to use multiplex PCR to determine if a sample contains a particular pathogen. However, since I am targeting the entire genome rather than specific regions, I would like to know if your tool is suitable for designing multiplex primers under these circumstances.
Thank you for your assistance. I look forward to your guidance.
Best wishes,
Zheng zhuqing