ShouWenWang-Lab / snakemake_DARLIN

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Custom configs in python pipeline and bulk python allele calling #11

Open jzussman opened 4 months ago

jzussman commented 4 months ago

Would adapting the existing python-based single-cell pipeline to the newer 10xV4 chemistry be as simple as adding in the 10xV4 barcode list to /reference? What else would be required to complete this configuration? I have successfully generated this config using the MATLAB CARLIN pipeline version, but am not sure how I would do so in the python version.

Would it be possible for you to provide a Bulk sequencing version of the python method for allele calling? Alternatively, if you don't have the bandwidth at the moment, could you instruct me on how I could most easily adapt the existing python pipeline to bulk sequence data?