j-andrews7
commented
3 years ago Nope, shouldn't matter on a cell by cell basis. Each cell is considered and scored individually.
On Wed, Jul 7, 2021, 6:08 PM lkqnaruto @.***> wrote:
I wonder whether the annotation result will be any different after I delete some cells. For example, originally I have 100k cells after QC, then I "LogNormalize" my data and use SingleR to assign cell labels based on a reference set. Then I use a subset of my original dataset, let's say 80k cells in total. Is it possible that a cell was assigned to cell type A at first, but then be assigned to cell type B ? In both case, reference set stays the same.
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I wonder whether the annotation result will be any different after I delete some cells. For example, originally I have 100k cells after QC, then I "LogNormalize" my data and use SingleR to assign cell labels based on a reference set. Then I use a subset of my original dataset, let's say 80k cells in total. Is it possible that a cell was assigned to cell type A at first, but then be assigned to cell type B ? In both case, reference set stays the same.