SlavovLab / EMT_TGFB_2023

Dynamics of single-cell protein covariation during EMT. Analysis of TGF-B induced EMT in MCF10A cells using nPoP and SCoPE2.
https://scp.slavovlab.net/Khan_et_al_2023
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Correction for cell_IDs #2

Open EnesSefaAyar opened 2 weeks ago

EnesSefaAyar commented 2 weeks ago

Thank you for sharing all the information. I was able to reproduce the exact results using your Matlab scripts but couldn't reproduce them in my R implementation. I noticed a mistake in your scripts:

Cell_Ids = txt(1,2:end);

The Cell_Ids are shifted, which affects the subsetting by conditions (d0, d3, and d9). The resulting clusters are quite different after correcting this mistake. I observed 2 distinct clusters instead of 3 and memberships are different with the ARI index (~0.25).

Correction: Cell_Ids = txt(1,1:end);

Best regards, Enes @nslavov