Using the FlowSOM plugin on FlowJo, I've generated MSTs for two sample types (both colored on a single marker of interest).
Both samples express the marker at different levels, but the MSTs are scaled and colored individually (one from ~200-550; the other from ~200-650). Accordingly, it is impossible to tell the difference between the samples by comparing color intensity alone (i.e., the min/max intensities are different, but they are both colored the same shades of blue/red).
Does anyone know if (and how) it is possible to modify the scale settings so they are the same (e.g., ~200-650) on both MSTs?
Using the FlowSOM plugin on FlowJo, I've generated MSTs for two sample types (both colored on a single marker of interest).
Both samples express the marker at different levels, but the MSTs are scaled and colored individually (one from ~200-550; the other from ~200-650). Accordingly, it is impossible to tell the difference between the samples by comparing color intensity alone (i.e., the min/max intensities are different, but they are both colored the same shades of blue/red).
Does anyone know if (and how) it is possible to modify the scale settings so they are the same (e.g., ~200-650) on both MSTs?
Any guidance would be gratefully accepted.
Best,
Ben