Expected copies of virus
[BCoV Stock] x vaccine vol. / WW volume
Measured copies of virus
[copies of BCoV] per ul RNA = [copies of BCoV] per reaction well / vol. template in reaction
[copies of BCoV] per ul RNA x F ul RNA/ ml WW x (1e3 ml to ul unit conversion)
Variable name references (plug into equations)
F = 1,000 = 50 ml WW/ 50 ul RNA
Vaccine vol = spike_virus_volume
Expected copies of virus
[BCoV Stock] x vaccine vol. / WW volume
Measured copies of virusVariable name references (plug into equations)