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StructuralGenomicsConsortium / CNP10-CHIKV-Naphthoquinone

An Open Chemistry Network dedicated to finding hits vs CHIKV based on a hit containing an naphthoquinone.
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Cytotoxicity concern #1

Closed alvaro-magalhaes closed 7 months ago

alvaro-magalhaes commented 10 months ago

It has been raised some concern regarding the structural similarity of these naphthoquinones to RA-0001914 - from DENV Rdrp ChEMBL series. RA-0001914 showed cytotoxicity when tested for human mitochondrial RNA production.

The naphthoquinones report show no cytotoxicity when tested in the following conditions: Quote from the paper "The naphthoquinone derivatives cytotoxicity in Vero cells was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide tetrazolium (MTT) reduction colorimetric assay in 96-well plate and incubated for 24 h at 37 °C.28 After, naphthoquinones derivatives were added in increasing concentrations, ranging from 50 to 1000 µM, diluted in DMEM. After this period, the cells were incubated with the MTT method and evaluated in a microplate reader to determine cell viability. DMSO-treated cells (1.0%) in DMEM were used as control of the evaluation. Determination of cell viability was calculated by percentage comparing treated cells with untreated cells and cells in DMSO" 10.21577/0103-5053.20220010.

It is important that cytotoxicity is evaluated alongside antiviral activity.

mattodd commented 7 months ago

Closing as these data have now been obtained, and this issue has been linked in the Story So Far.