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Sun-lab / ideas

Individual level Differential Expression Analysis for Single cells
MIT License
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Number of replicates less than 5 #2

Open torgheh opened 2 years ago

torgheh commented 2 years ago

Hi,

Thanks for your amazing work.

I was wondering if you know any method that can reliably produce p-values using the ideas' distance when number of replicates in each group in less than 5? Since I see in your permanova method the minimum number of replicates is 5.

Thanks.

sunway1999 commented 2 years ago

when the sample size in each group is smaller than 5, the total number of permutations you can get is small. For example, if you have 4 cases vs. 4 controls, the total number of permutation is 70, and the smallest p-value you can get is 1/70, also the distribution of permutation p-value can be unstable. so it is doable, but not recommended.