wgdi can not recognize the PATH of mafft

[tinyfallen]

Dear developer,

I met the following issue using wgdi to calculate the ks and need some help. Looking forward to your reply, thanks!

cds_file = tsin.cds pep_file = tsin.prot align_software = muscle pairs_file = icl.txt ks_file = ks.txt Traceback (most recent call last): File "/export/home/ydn/.conda/envs/cg/bin/wgdi", line 8, in sys.exit(main()) File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/run.py", line 158, in main module_to_run(arg, value) File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/run.py", line 118, in module_to_run run_subprogram(program, conf, name) File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/run.py", line 84, in run_subprogram r.run() File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/ks.py", line 101, in run kaks = self.pair_kaks(k) File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/ks.py", line 113, in pair_kaks self.align() File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/ks.py", line 134, in align stdout, stderr = muscle_cline() File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/Bio/Application/init.py", line 574, in call raise ApplicationError(return_code, str(self), stdout_str, stderr_str) Bio.Application.ApplicationError: Non-zero return code 127 from 'C:\bio\muscle3.8.31_i86win32.exe -in pair.pep -out prot.aln -seqtype protein -clwstrict', message '/bin/sh: C:biomuscle3.8.31_i86win32.exe: command not found'

Traceback (most recent call last): File "/export/home/ydn/.conda/envs/cg/bin/wgdi", line 8, in sys.exit(main()) File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/run.py", line 158, in main module_to_run(arg, value) File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/run.py", line 118, in module_to_run run_subprogram(program, conf, name) File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/run.py", line 84, in run_subprogram r.run() File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/ks.py", line 101, in run kaks = self.pair_kaks(k) File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/ks.py", line 113, in pair_kaks self.align() File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/wgdi/ks.py", line 128, in align stdout, stderr = mafft_cline() File "/export/home/ydn/.conda/envs/cg/lib/python3.9/site-packages/Bio/Application/init.py", line 574, in call raise ApplicationError(return_code, str(self), stdout_str, stderr_str) Bio.Application.ApplicationError: Non-zero return code 127 from 'C:\bio\mafft-win\mafft.bat --auto pair.pep', message '/bin/sh: C:biomafft-winmafft.bat: command not found'

[tinyfallen]

Sorry for disturbing, I found the description at the installation guide