Swart-lab / bleties

IES retention analysis from PacBio CCS reads
https://swart-lab.github.io/bleties
MIT License
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Retention scores systematically underestimated #28

Open kbseah opened 3 years ago

kbseah commented 3 years ago

Both MILRET and MILRAA count only reads that completely span an IES and either contain an insert or do not. If inserts/IESs are too long, the mapping will have a clip instead of an insert. For short IESs this is negligible, but as IESs are longer, a greater proportion of mapped IES+ reads will have clips, but these are currently ignored in calculating the retention score.