If fastq split is run >1 on same input without cleaning Fastq_split output directory, some reads can end up duplicated because fastp does not always produce the same filepaths for outputs between runs. A cleanup is necessary between re-runs of fastq split.
calizilla
commented
1 day ago
If fastq split is run >1 on same input without cleaning Fastq_split output directory, some reads can end up duplicated because fastp does not always produce the same filepaths for outputs between runs. A cleanup is necessary between re-runs of fastq split.