Closed fransamsing closed 2 months ago
The following error message arised when running new data (Super Accuracy Basecalling)
> ERROR ~ Error executing process > 'unicycler_assembly (ASSEMBLING GENOME WITH UNICYCLER: barcode08)' > > Caused by: > Process `unicycler_assembly (ASSEMBLING GENOME WITH UNICYCLER: barcode08)` terminated with an error exit status (137) > > > Command executed: > > unicycler --long barcode08_trimmed.fastq.gz \ > --threads 8 \ > --out barcode08_unicycler_assembly > > Command exit status: > 137 > > Command output: > 252,887 / 428,622 (59.0%) - 963,803,473 bp > 257,174 / 428,622 (60.0%) - 979,748,723 bp > 261,460 / 428,622 (61.0%) - 996,087,674 bp > 265,746 / 428,622 (62.0%) - 1,012,332,484 bp > 270,032 / 428,622 (63.0%) - 1,028,890,234 bp > 274,319 / 428,622 (64.0%) - 1,049,729,213 bp > 278,605 / 428,622 (65.0%) - 1,066,149,790 bp > 282,891 / 428,622 (66.0%) - 1,083,010,854 bp > 287,177 / 428,622 (67.0%) - 1,099,443,189 bp > 291,463 / 428,622 (68.0%) - 1,115,531,160 bp > 295,750 / 428,622 (69.0%) - 1,132,152,042 bp > 300,036 / 428,622 (70.0%) - 1,147,351,328 bp > 304,322 / 428,622 (71.0%) - 1,162,752,261 bp > 308,608 / 428,622 (72.0%) - 1,178,793,477 bp > 312,895 / 428,622 (73.0%) - 1,195,043,124 bp > 317,181 / 428,622 (74.0%) - 1,211,837,697 bp > 321,467 / 428,622 (75.0%) - 1,227,733,457 bp > 325,753 / 428,622 (76.0%) - 1,243,719,464 bp > 330,039 / 428,622 (77.0%) - 1,260,174,223 bp > 334,326 / 428,622 (78.0%) - 1,276,061,255 bp > 338,612 / 428,622 (79.0%) - 1,292,689,204 bp > 342,898 / 428,622 (80.0%) - 1,309,268,643 bp > 347,184 / 428,622 (81.0%) - 1,325,508,429 bp > 351,471 / 428,622 (82.0%) - 1,341,041,649 bp > 355,757 / 428,622 (83.0%) - 1,357,017,425 bp > 360,043 / 428,622 (84.0%) - 1,373,072,028 bp > 364,329 / 428,622 (85.0%) - 1,389,147,760 bp > 368,615 / 428,622 (86.0%) - 1,404,235,054 bp > 372,902 / 428,622 (87.0%) - 1,419,617,744 bp > 377,188 / 428,622 (88.0%) - 1,435,659,978 bp > 381,474 / 428,622 (89.0%) - 1,451,685,308 bp > 385,760 / 428,622 (90.0%) - 1,468,099,130 bp > 390,047 / 428,622 (91.0%) - 1,483,947,895 bp > 394,333 / 428,622 (92.0%) - 1,500,080,390 bp > 398,619 / 428,622 (93.0%) - 1,516,519,200 bp > 402,905 / 428,622 (94.0%) - 1,532,438,465 bp > 407,191 / 428,622 (95.0%) - 1,547,932,555 bp > 411,478 / 428,622 (96.0%) - 1,564,026,090 bp > 415,764 / 428,622 (97.0%) - 1,580,706,080 bp > 420,050 / 428,622 (98.0%) - 1,596,392,683 bp > 424,336 / 428,622 (99.0%) - 1,612,505,890 bp > 428,622 / 428,622 (100.0%) - 1,629,048,883 bp > 428,622 / 428,622 (100.0%) - 1,629,048,883 bp > > > Assembling contigs and long reads with miniasm (2024-08-12 14:37:39) > Saving to barcode08_unicycler_assembly/miniasm_assembly/01_assembly_reads.fastq: > 428,622 long reads > > Finding overlaps with minimap... > > Command error: > WARNING: Skipping mount /opt/nci/singularity/3.11.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container > tput: No value for $TERM and no -T specified > tput: No value for $TERM and no -T specified > tput: No value for $TERM and no -T specified > .command.sh: line 4: 2898183 Killed unicycler --long barcode08_trimmed.fastq.gz --threads 8 --out barcode08_unicycler_assembly > > Work dir: > /scratch/tj48/fs9163/ONT-bacpac-nf/work/e5/d627965180ee1f2bd0268576cb3c9b > > Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out` > > -- Check '.nextflow.log' file for details
To Reproduce Steps to reproduce the behavior:
bash test/run_test.sh
**Environment (please complete the following information):
@fransamsing, I've significantly increased the memory allocated to Unicycler. Want to give it another go?
The following error message arised when running new data (Super Accuracy Basecalling)
To Reproduce Steps to reproduce the behavior:
bash test/run_test.sh
**Environment (please complete the following information):