cjmyers
commented
1 year ago We are doing sequence search using Vsearch (https://vcru.wisc.edu/simonlab/bioinformatics/programs/vsearch/vsearch_manual.pdf). We have also struggled to figure out how to configure it to give "close" but not "exact" results. If you have any suggestions, please let us know.
When using the sequence match feature, results only appear when there is a 100% sequence match. So my input looks like this:
https://synbiohub.org/search/sequence=<seq>&The results only return on 100% match. For examplehttps://synbiohub.org/search/globalsequence=atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa&id=1.0&Returns Matches but:https://synbiohub.org/search/globalsequence=ttgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa&id=1.0&Will not. It seems changing the ID value doesn't make a difference. Is this an incorrect way of using the API, or is it a bug?