AJamesPhillips
commented
9 years ago When the algorithm is run now it finds the following (note includes both forward and reverse universal primers):
U-fwd primer
position: 891
sequence: TGCCACCTGACGTCTAAGAA
Fwd-1 primer
position: 1300
sequence: GTCTGCTTGTCCACCCGTAC
Fwd-2 primer
position: 1720
sequence: TCTGCCTCTCTCTGCAACTA
Fwd-3 primer
position: 2137
sequence: TCTGTCTGGGAAAATCAGGTAAC
Fwd-4 primer
position: 2560
sequence: ACTACGCTCCAGAGCCAAAT
Fwd-5 primer
position: 2934
sequence: GCATGTGGACAGTATCCTTCAAAA
Fwd-6 primer
position: 3357
sequence: CCGGTATCAGCTCACTCAAAG
Fwd-7 primer
position: 3773
sequence: CTTATCCGGTAACTATCGTCTTGAG
U-rvs primer
position: 3366
sequence: ATTACCGCCTTTGAGTGAGC (forward strand: GCTCACTCAAAGGCGGTAAT)
Rvs-2 primer
position: 2994
sequence: CTTGAGGGTCCCTATGTCCC (forward strand: GGGACATAGGGACCCTCAAG)
Rvs-3 primer
position: 2781
sequence: CATGACAGCCAGTATCCAGGATTG (forward strand: CAATCCTGGATACTGGCTGTCATG)
Rvs-4 primer
position: 2361
sequence: AAGCAAAAAGGGGGACAGAC (forward strand: GTCTGTCCCCCTTTTTGCTT)
Rvs-5 primer
position: 1997
sequence: GGGTGGGTTAAATTGTCTGTTTCAA (forward strand: TTGAAACAGACAATTTAACCCACCC)
Rvs-6 primer
position: 1577
sequence: TCAAAGAAGCGGTCCTTATCAG (forward strand: CTGATAAGGACCGCTTCTTTGA)
Rvs-7 primer
position: 1157
sequence: CCAAAGGCAGATCATCATCACTT (forward strand: AAGTGATGATGATCTGCCTTTGG)
Rvs-8 primer
position: 737
sequence: TGGATATACCACCGTTGATATATCCC (forward strand: GGGATATATCAACGGTGGTATATCCA)
Rvs-9 primer
position: 318
sequence: ATCCCTGGGTGAGTTTCACC (forward strand: GGTGAAACTCACCCAGGGAT)
This is a circuit built from parts we're giving to iGEM teams. There's no reverse universal sequencing primer found.
[Can't seem to attach SYBIL files. I've cut and pasted this.]
TGAGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGTGCCACCTGACGTCTAAGAAACCAGAATTCTCATGACATTAACCTCCAGTAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGTTGTTCAGAACGCTCGGTCTTGCACACCGGGCGTTTTTTCTTTGTGAGTCCAGCGGCCGCGTGAGGTCAGATGCTGAACGTCGGGTCTCAGATGTTTACGGCTAGCTCAGTCCTAGGTACTATCGGCTGAGTACCTCACACAGGAAAGTGATGATGATCTGCCTTTGGGTCCTCTGTTTCGTCCTTTTCCCCTACTCCTTGTCCTATCCCGAGAGCTGGATGCCCCTCATAAACCTAACCCACCATATCCTGCGTGATACCAACTCTTCCTTTTTTCCCAACTGCTGGGTCTGCTTGTCCACCCGTACCCAGCGCTCCCTAGCGGTCCCAGCTCCTTTGTCCACTTGGACAGACTCACCCATGAAGCTTCACCTCACGTACTCAGTCAAGGCCTTTTCTGGCTCTTATCCCATCACTGACATTGAGAGGCGCCTCCAACTCTTCCACCCGTTGACTGCCTCCTATTCTTTCCACAATCCTGACCGACGGGCTATTGCTTTCCTTCAGCTTGTCAGTTCGACAGGCATATTCCCAATCATCACCCGACTCACCTCTGTGATATATCCTGATAAGGACCGCTTCTTTGAGTCTGCCCAGCGCCCTTTATGGGGACCACTCTTTACTGAGACGCTCCTCATGTCACAGGCTCCGCTCTGCATATCCCGCTCCTTCAAGGTCCCAGCTTACGCCACTTTTGTAGGCACGCTCTCTGCCTCTCTCTGCAACTACACTCTTCATATATCACCTTCCAATAGTCATGTAAACCTAGATCTGTCCACTACCCATCTATTCAAACAGGCAATGAAAAGACCAGATGCCAAATGGAAAAATCCCCTCCGCTTTTCGGGACCTCCCTCTCTAGTCTCCTCAGAACGTTTTTACTATCCATGCCCAACTGACATCAAACACTGTCATACCTCCCCAGCCACTCCCTGGATGCGCTGTCCTCTCTCCCCGTCTGGCACCTGCTATAATCTCACTCTATTTGAAACAGACAATTTAACCCACCCTGTTACTATGTCAGTGAACCCTACCTACTTCAAGCTCAAACTCCAGGACATAGAAACCCTATCCACTCTCCCACTACCAGCCCCTCAAATAGGAGCTGCGCTGTCTGGACAATATTCTGTCTGGGAAAATCAGGTAACTATTCAAGAAAACTGGGGTGTCACCTCAAATACATTCTCCCATCTCCTCACTTTCTCATACTCCTTCTGTCTCAACTCTTCAGGTGTTTTCTTCCTCTGTGGAACATCAACTTACATGTGTCTCCCGGCCAATTGGTCCGGGGTCTGTACCCTTGTCTTCCAATACCCAGACATCGAACTTCTCCCCAATCACCAAACAGTGTCTGTCCCCCTTTTTGCTTCAGTTCGTGCCTCAGCTTCTCACCCAAAGAGGTCCCCTCACCTCTTCCCCTTCCTTGCAGGCCTGGGCATCTCTTCTGCCCTTGGTACTGGTATAGCTGGCTTGGCCTCCTCAACTTTTTATTTCCAGCGGCTTTCGAAGGCTCTTTCGGACACCCTGGATCAAATAGCCGCCTCTGTCACTACGCTCCAGAGCCAAATAGACTCGCTGGCGGGTGTCGTTCTTCAAAACCGCAGGGCGCTGGACCTCATCGCAGCCGAGAAAGGGGGCACCTGTCTCTTTCTCCAAGAAGAGTGCTGCTTCTATGTAAACCAGTCTGGAATAGTCCGAGATGCTGCAAGGAAACTCCGAGAACGAGCATCAGAACTTGGCCAGCATTCTCACTCTTGGGGACAGTGGCCCAATCCTGGATACTGGCTGTCATGGCTGACTCCCTTCCTGGGACCTCTCCTCTTCATTGTCTTCCTATTGACATTTGGGTCTTGTCTGCTTCACTGCCTAACCCGTTTTGTGTCCCGGAGACTTGGCTCCTTTGTCCAAGATGCCACCAAAAGGCATGTGGACAGTATCCTTCAAAATGTCCACTATCAAAGACTGCCCCAAGATGCCCCAACGGGACATAGGGACCCTCAAGACACCGCTCCTGTGTACCAGGGAGAAGTTGGGAGAGCACCAGCGTTGTCCTCCTTACTTTCCAGTTCATACGAAACCAAGTCGGGTGTTAGTCATTGCCACAGTGTTTTGTATCCATCACAATTCCAGAAACGTAATTTTGCCTCCAGAACTAACATTACTCACTTTTCCTGACGGCTGAGACCCGTACCGCTGTCCAAGCGAAACTAGCGGCCGCGTCCGGCAAAAAAACGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCATGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCCCTCAGCTTTTTTTTTTTTTTTTTTTTGC