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SysBioChalmers / Human-GEM

The generic genome-scale metabolic model of Homo sapiens
https://sysbiochalmers.github.io/Human-GEM-guide/
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GPR curation for Estrogen metabolism/Steroid metabolism #851

Open JHL-452b opened 1 month ago

JHL-452b commented 1 month ago

Current behavior:

45 genes are discovered to be uncorrelated with their corresponding reactions in Estrogen metabolism/Steroid metabolism

Gene UniProtID Reaction GPR Correlation Manually check
ENSG00000119723 Q9Y2Z9 MAR01927: cholesterol + H+ + NADPH + O2 --> 22beta-hydroxycholesterol + H2O + NADP+ ENSG00000119723 or ENSG00000130649 or ENSG00000145476 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000119723 encodes for a FAD-dependent monooxygenase involved in C5-ring hydroxylation during ubiquinone biosynthesis, specifically hydroxylating 3-hexaprenyl-4-hydroxybenzoic acid to 3-hexaprenyl-4,5-dihydroxybenzoic acid. The provided reaction involves cholesterol hydroxylation, which is not related to the ubiquinone biosynthesis pathway in which the gene is involved. Thus, the gene and the reaction are unrelated. No
ENSG00000145476 Q6ZWL3 MAR01927: cholesterol + H+ + NADPH + O2 --> 22beta-hydroxycholesterol + H2O + NADP+ ENSG00000119723 or ENSG00000130649 or ENSG00000145476 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000145476 encodes a cytochrome P450 monooxygenase involved in the omega-hydroxylation of polyunsaturated and saturated fatty acids in the eye. It catalyzes reactions involving laurate, myristate, palmitate, eicosapentaenoate (EPA), and docosahexaenoate (DHA), but not cholesterol. The provided reaction involves the hydroxylation of cholesterol to form 22beta-hydroxycholesterol, which is not a substrate or product of the reactions catalyzed by the gene's encoded enzyme according to the provided annotations. Hence, the gene is not related to the reaction involving cholesterol. No
ENSG00000186377 Q8N118 MAR02029: 2.0 H+ + O2 + testosterone --> 19-hydroxytestosterone + H2O ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 codes for a cytochrome P450 monooxygenase that specifically catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide but has no hydroxylase activity toward various fatty acids, steroids, and prostaglandins. The provided reaction involves the conversion of testosterone to 19-hydroxytestosterone, which implies hydroxylase activity towards a steroid, specifically testosterone. Since the gene's product does not exhibit hydroxylase activity and instead modulates endocannabinoid signaling through epoxidation of anandamide, it is not relevant to the hydroxylation reaction involving testosterone. No
ENSG00000108242 P33260 MAR02029: 2.0 H+ + O2 + testosterone --> 19-hydroxytestosterone + H2O ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000108242 encodes a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid to 4-hydroxyretinoate. The provided reaction involves the conversion of testosterone to 19-hydroxytestosterone, a different substrate and reaction pathway. Therefore, based on the gene's annotation, the gene and the testosterone hydroxylation reaction are not related. No
ENSG00000155016 Q7Z449 MAR02029: 2.0 H+ + O2 + testosterone --> 19-hydroxytestosterone + H2O ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. Based on the annotation of the gene ENSG00000155016, it primarily functions as a cytochrome P450 monooxygenase involved in metabolizing arachidonic acid and other long-chain fatty acids. The provided reaction involves the conversion of testosterone to 19-hydroxytestosterone, which is not mentioned among the enzymatic activities or specific substrates associated with this gene in the annotations provided. Therefore, the gene ENSG00000155016 does not seem to participate in or influence the given reaction. No
ENSG00000186526 P98187 MAR02029: 2.0 H+ + O2 + testosterone --> 19-hydroxytestosterone + H2O ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The provided gene, ENSG00000186526, encodes a cytochrome P450 monooxygenase that is involved in the metabolism of polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives, such as hydroxylating arachidonate and metabolizing prostaglandins and docosahexaenoic acids. However, the reaction provided involves the conversion of testosterone to 19-hydroxytestosterone. The annotation for the gene does not mention any activity related to the metabolism of testosterone. Therefore, the gene and the reaction are not related. No
ENSG00000186160 Q86W10 MAR02031: 19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186160 encodes a cytochrome P450 monooxygenase, which primarily catalyzes the oxidation of fatty acids, such as the hydroxylation of lauric and myristic acids and the epoxidation of arachidonic acid. The provided reaction involves the conversion of 19-oxo-testosterone to estradiol-17beta, which is not within the enzymatic activity scope of this cytochrome P450 enzyme that specializes in fatty acid metabolism. Therefore, the gene does not relate to this specific reaction involving steroid transformation. No
ENSG00000186204 Q9HCS2 MAR02031: 19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186204 encodes for a cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFAs) and the metabolism of xenobiotics. Its catalytic activities include hydroxylation and epoxidation of PUFAs and certain drugs, but there is no mention of its involvement in the reaction converting 19-oxo-testosterone and NADPH to estradiol-17beta, formate, H2O, and NADP+. Therefore, the gene is not related to the provided reaction. No
ENSG00000155016 Q7Z449 MAR02031: 19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000155016 encodes a cytochrome P450 monooxygenase involved in the metabolism of arachidonic acid and other long chain fatty acids, catalyzing their hydroxylation and oxidation. However, the reaction specified ¡ª 19-oxo-testosterone + NADPH + O2 -> estradiol-17beta + formate + H2O + NADP+ ¡ª involves the conversion of 19-oxo-testosterone to estradiol-17beta, which is not related to the known substrates or catalytic activities of this cytochrome P450. Therefore, the gene and the provided reaction are not related. No
ENSG00000186377 Q8N118 MAR02031: 19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that specifically catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide. It does not have hydroxylase activity toward various fatty acids, steroids, and prostaglandins, and it uses molecular oxygen and NADPH via cytochrome P450 reductase for its catalytic activity. In contrast, the provided reaction involves the conversion of 19-oxo-testosterone into estradiol-17beta, which does not align with the specific function and activity of the gene product described. No
ENSG00000167600 Q96SQ9 MAR02031: 19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000167600 encodes a cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids. The listed catalytic activities include the metabolism of all-trans-retinoate and various hydroperoxyeicosatetraenoates but do not involve the conversion of 19-oxo-testosterone to estradiol-17beta. The described activities require substrates like all-trans-retinoate, PGH2, and hydroperoxyeicosatetraenoates, which are different from the substrates and products in the provided reaction involving 19-oxo-testosterone. Therefore, based on the gene's annotation, there is no connection between the gene ENSG00000167600 and the specific reaction provided. No
ENSG00000134716 P51589 MAR02031: 19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000134716 encodes a cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) and the epoxidation of double bonds of PUFA. It mainly converts arachidonic acid into various epoxyeicosatrienoic acids (EpETrE) and also metabolizes xenobiotics. However, the reaction 19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+ pertains to the conversion of 19-oxo-testosterone to estradiol-17beta, a transformation that is not related to the described functions of the gene product. Therefore, this gene is not relevant to the provided reaction. No
ENSG00000186526 P98187 MAR02031: 19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186526 encodes a cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives. It catalyzes the hydroxylation and epoxidation of PUFAs using molecular oxygen and NADPH. However, the reaction provided (19-oxo-testosterone + NADPH + O2 --> estradiol-17beta + formate + H2O + NADP+) involves the conversion of 19-oxo-testosterone to estradiol-17beta, which is a different type of reaction involving steroid metabolism, not related to the metabolic functions and substrates (PUFAs and oxylipins) mentioned for the gene. Therefore, based on the annotation, the gene and the reaction are not related. No
ENSG00000186377 Q8N118 MAR02032: 4-androstene-3,17-dione + 2.0 H+ + O2 --> 19-hydroxyandrostenedione + H2O ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide. It does not exhibit hydroxylase activity toward fatty acids, steroids, and prostaglandins. The provided reaction involves the hydroxylation of 4-androstene-3,17-dione to produce 19-hydroxyandrostenedione, which is not catalyzed by the enzyme encoded by this gene. Thus, the gene is not relevant to the reaction provided. No
ENSG00000108242 P33260 MAR02032: 4-androstene-3,17-dione + 2.0 H+ + O2 --> 19-hydroxyandrostenedione + H2O ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000108242 encodes a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid (atRA) to 4-hydroxyretinoate. The provided reaction involves the conversion of 4-androstene-3,17-dione to 19-hydroxyandrostenedione, which does not relate to retinoid metabolism or the function, substrates, or catalytic activities described for the gene's product. Therefore, the gene and the reaction are not related. No
ENSG00000108242 P33260 MAR02033: 19-hydroxyandrostenedione + 2.0 H+ + O2 --> 19-oxoandrostenedione + 2.0 H2O ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000108242 encodes for a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid to 4-hydroxyretinoate. The reaction provided involves the conversion of 19-hydroxyandrostenedione to 19-oxoandrostenedione, which is not related to retinoid metabolism. Therefore, based on the gene's annotated function and catalytic activities, it does not participate in the reaction mentioned. No
ENSG00000108242 P33260 MAR02034: 19-oxoandrostenedione + NADPH + O2 --> estrone + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000108242 encodes a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid to 4-hydroxyretinoate. Its catalytic activities involve inserting oxygen into retinoids using NADPH as an electron donor. However, the reaction provided involves converting 19-oxoandrostenedione to estrone with NADPH and O2, a process not related to retinoid metabolism. Thus, the gene's function and catalytic activities do not align with the reaction described. No
ENSG00000186526 P98187 MAR02034: 19-oxoandrostenedione + NADPH + O2 --> estrone + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186526 encodes for a cytochrome P450 monooxygenase, which primarily catalyzes the hydroxylation and epoxidation of polyunsaturated fatty acids (PUFAs) and their derivatives, such as arachidonate, prostaglandins, and docosahexaenoic and docosapentaenoic acids, utilizing NADPH and oxygen. The reaction provided, 19-oxoandrostenedione + NADPH + O2 --> estrone + formate + H2O + NADP+, involves the conversion of 19-oxoandrostenedione to estrone, which does not fall within the range of substrates or reaction types (hydroxylation and epoxidation of PUFAs) catalyzed by this gene's enzyme product. Therefore, based on the functional annotation, this gene is not relevant to the given reaction. No
ENSG00000186204 Q9HCS2 MAR02034: 19-oxoandrostenedione + NADPH + O2 --> estrone + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The described gene ENSG00000186204 encodes a cytochrome P450 monooxygenase involved in the hydroxylation and epoxidation of polyunsaturated fatty acids, and the metabolism of xenobiotics with specific catalytic activities involving NADPH and molecular oxygen. However, the reaction provided (19-oxoandrostenedione + NADPH + O2 --> estrone + formate + H2O + NADP+) involves the conversion of an androgen to an estrogen, which is not covered by the described activities of the gene ENSG00000186204. No
ENSG00000186377 Q8N118 MAR02034: 19-oxoandrostenedione + NADPH + O2 --> estrone + formate + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes for a cytochrome P450 monooxygenase that catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, modulating endocannabinoid signaling. This enzyme specifically uses molecular oxygen and NADPH to perform this reaction. However, the reaction provided involves the conversion of 19-oxoandrostenedione to estrone, formate, water, and NADP+, which is a different biochemical process that the annotated gene's enzyme does not catalyze or participate in. Hence, the gene and the reaction are not related. No
ENSG00000157399 P51690 MAR02038: estrone 3-sulfate + H2O --> estrone + H+ + sulfate ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000157399 exhibits arylsulfatase activity and has no activity toward steroid sulfates, whereas the reaction provided involves the hydrolysis of estrone 3-sulfate, which is a steroid sulfate. This functional characteristic of the gene makes it irrelevant to the reaction involving estrone 3-sulfate. No
ENSG00000155016 Q7Z449 MAR02042: estradiol-17beta + H+ + NADPH + O2 --> 2-hydroxyestradiol-17beta + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000155016 is involved in the metabolism of arachidonic acid and its conjugates, using molecular oxygen to insert one oxygen atom into substrates like long-chain fatty acids and N-arachidonoyl-serotonin. The catalytic activities listed involve reactions with specific substrates such as long-chain fatty acids and N-arachidonoyl-serotonin, none of which include estradiol-17beta. The provided reaction involves the hydroxylation of estradiol-17beta, which is not among the reactions catalyzed by the gene product according to the provided annotations. Therefore, the gene is not relevant to the reaction involving estradiol-17beta. No
ENSG00000186377 Q8N118 MAR02042: estradiol-17beta + H+ + NADPH + O2 --> 2-hydroxyestradiol-17beta + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that specifically catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide. It does not exhibit hydroxylase activity toward various fatty acids, steroids, and prostaglandins. The detailed catalytic activity described involves converting N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine to N-(14,15-epoxy-5Z,8Z,11Z-eicosatrienoyl)-ethanolamine using molecular oxygen and NADPH via cytochrome P450 reductase. The reaction provided (estradiol-17beta + H+ + NADPH + O2 --> 2-hydroxyestradiol-17beta + H2O + NADP+) involves hydroxylation of estradiol-17beta, which is not within the functional activity of the specified gene, hence they are not related. No
ENSG00000186526 P98187 MAR02042: estradiol-17beta + H+ + NADPH + O2 --> 2-hydroxyestradiol-17beta + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186526 encodes a cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives. It predominantly catalyzes hydroxylation and epoxidation reactions on substrates like eicosatetraenoic acid, docosahexaenoic, and prostaglandins. However, the provided reaction involves the hydroxylation of estradiol-17beta to 2-hydroxyestradiol-17beta, which is not among the substrates or reaction types described for this gene, indicating no direct relevance. No
ENSG00000108242 P33260 MAR02043: estradiol-17beta + H+ + NADPH + O2 --> 2-hydroxyestradiol-17beta + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000108242 encodes a cytochrome P450 monooxygenase that is involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid to 4-hydroxyretinoate. In contrast, the provided reaction involves the hydroxylation of estradiol-17beta to 2-hydroxyestradiol-17beta. Since the enzyme encoded by ENSG00000108242 is specific to retinoid substrates and not estradiol, the gene does not relate to the provided reaction. No
ENSG00000134716 P51589 MAR02043: estradiol-17beta + H+ + NADPH + O2 --> 2-hydroxyestradiol-17beta + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000134716 functions as a cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFA) and various xenobiotics. It catalyzes the epoxidation of PUFA and the hydroxylation and sulfoxidation of certain xenobiotics. However, the provided reaction involves the conversion of estradiol-17beta to 2-hydroxyestradiol-17beta, which is not among the reactions listed for this gene. Therefore, the gene is not relevant to the estradiol-17beta hydroxylation reaction. No
ENSG00000186377 Q8N118 MAR02043: estradiol-17beta + H+ + NADPH + O2 --> 2-hydroxyestradiol-17beta + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide. It does not exhibit hydroxylase activity toward various fatty acids, steroids, and prostaglandins. The catalytic activity described for the gene involves converting anandamide into a specific epoxide using molecular oxygen and NADPH via cytochrome P450 reductase. In contrast, the provided reaction involves the conversion of estradiol-17beta to 2-hydroxyestradiol-17beta, which is outside the scope of the enzyme's annotated activities. Thus, the gene and the reaction are not related. No
ENSG00000186377 Q8N118 MAR02053: estrone + H+ + NADPH + O2 --> 16alpha-hydroxyestrone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that catalyzes the epoxidation of the arachidonoyl moiety of anandamide, specifically without hydroxylase activity toward fatty acids, steroids, and prostaglandins. The provided reaction involves the conversion of estrone to 16alpha-hydroxyestrone, which implies hydroxylase activity on a steroid molecule. This hydroxylase function is not within the specific catalytic activities of the gene product mentioned. No
ENSG00000186160 Q86W10 MAR02053: estrone + H+ + NADPH + O2 --> 16alpha-hydroxyestrone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186160 encodes a cytochrome P450 monooxygenase that primarily catalyzes the in-chain oxidation and hydroxylation of fatty acids, as well as the epoxidation of polyunsaturated fatty acids. However, the mentioned reaction involves the conversion of estrone to 16alpha-hydroxyestrone, which pertains to estrogen metabolism and is not associated with the gene's annotated functions of fatty acid and PUFA metabolism. Therefore, the gene and the reaction are not related. No
ENSG00000186204 Q9HCS2 MAR02053: estrone + H+ + NADPH + O2 --> 16alpha-hydroxyestrone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186204 encodes a cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFAs) and xenobiotics by catalyzing hydroxylation and epoxidation reactions primarily on PUFAs and some other organic molecules. The specific activities described include metabolizing eicosatetraenoic, docosapentaenoic, and docosahexaenoic acids, as well as hydroxylation of the antihistamine drug ebastine. However, the provided reaction involves the conversion of estrone to 16alpha-hydroxyestrone, which is not listed among the substrates or reactions catalyzed by the cytochrome P450 monooxygenase enzyme encoded by ENSG00000186204. Thus, it is not relevant to the reaction provided. No
ENSG00000108242 P33260 MAR02053: estrone + H+ + NADPH + O2 --> 16alpha-hydroxyestrone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000108242 encodes a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid to 4-hydroxyretinoate using molecular oxygen and NADPH. The reaction provided involves the conversion of estrone to 16alpha-hydroxyestrone, which is not related to retinoid metabolism or the specific enzymatic activity described for ENSG00000108242. Therefore, they are not related. No
ENSG00000186204 Q9HCS2 MAR02054: estrone + H+ + NADPH + O2 --> 16alpha-hydroxyestrone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186204 encodes for a cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs) and xenobiotics. It catalyzes the hydroxylation and epoxidation of various fatty acids but has no known activity related to the hydroxylation of estrone. The provided reaction involves the conversion of estrone to 16alpha-hydroxyestrone, which is not among the catalytic activities associated with the gene described. Therefore, the gene and the reaction are not related. No
ENSG00000155016 Q7Z449 MAR02054: estrone + H+ + NADPH + O2 --> 16alpha-hydroxyestrone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The given gene, ENSG00000155016, encodes for a cytochrome P450 monooxygenase which is involved in the metabolism of arachidonic acid and its conjugates, acting as an omega and omega-1 hydroxylase for arachidonic acid and other long chain fatty acids. It also catalyzes the C-2 oxidation of the indole ring of N-arachidonoyl-serotonin. However, the provided reaction involves the conversion of estrone to 16alpha-hydroxyestrone, which is not related to the metabolism of arachidonic acid or its related pathways. Therefore, based on the annotation, this gene and the specified reaction are not related. No
ENSG00000186377 Q8N118 MAR02054: estrone + H+ + NADPH + O2 --> 16alpha-hydroxyestrone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide. This enzyme specifically catalyzes the reaction involving N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine and molecular oxygen, producing N-(14,15-epoxy-5Z,8Z,11Z-eicosatrienoyl)-ethanolamine. It does not exhibit hydroxylase activity toward fatty acids, steroids, or prostaglandins. Conversely, the reaction provided involves the conversion of estrone to 16alpha-hydroxyestrone, which is not within the functional capabilities of the enzyme encoded by ENSG00000186377. Therefore, the gene and the reaction are not related. No
ENSG00000186526 P98187 MAR02054: estrone + H+ + NADPH + O2 --> 16alpha-hydroxyestrone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186526 encodes a cytochrome P450 monooxygenase that primarily metabolizes endogenous polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives (oxylipins). It catalyzes hydroxylation and epoxidation reactions involving PUFAs and prostaglandins. However, the reaction involving the conversion of estrone to 16alpha-hydroxyestrone is a specific estrogen metabolism reaction and is not related to the functions or catalytic activities described for this gene. No
ENSG00000115705 P07202 MAR02077: 4-hydroxyestrone + H+ + H2O2 <=> estrone-3,4-semiquinone + 2.0 H2O ENSG00000005381 or ENSG00000115705 or ENSG00000117592 or ENSG00000121053 or ENSG00000167419 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000115705 is involved in the iodination and coupling of hormonogenic tyrosines in thyroglobulin to produce thyroid hormones T(3) and T(4). Its catalytic activities are associated with reactions involving thyroglobulin and iodide, leading to the production of iodinated tyrosines and thyroid hormones. However, the reaction provided involves the transformation of 4-hydroxyestrone to estrone-3,4-semiquinone using H2O2 and H+, which is unrelated to the processes described for this gene. Therefore, the gene is not relevant to the reaction provided. No
ENSG00000115705 P07202 MAR02089: 17beta-estradiol-3,4-semiquinone + 2.0 H2O <=> 4-hydroxy-17beta-estradiol + H2O2 ENSG00000005381 or ENSG00000115705 or ENSG00000117592 or ENSG00000121053 or ENSG00000167419 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000115705 is associated with the iodination and coupling of hormonogenic tyrosines in thyroglobulin to yield thyroid hormones T3 and T4. Its catalytic activities involve reactions such as iodination of tyrosine residues in thyroglobulin and the formation of thyroid hormones. However, the provided reaction involves the conversion of 17beta-estradiol-3,4-semiquinone to 4-hydroxy-17beta-estradiol with the production of H2O2, which is unrelated to the iodination processes performed by the gene ENSG00000115705. No
ENSG00000186526 P98187 MAR07940: 4-androstene-3,17-dione + NADPH + 2.0 O2 --> estrone + formate + 2.0 H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186526 encodes a cytochrome P450 monooxygenase that catalyzes the hydroxylation and epoxidation of various polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives. It specifically functions by using molecular oxygen and NADPH to insert an oxygen atom into substrates, predominantly hydroxylating and epoxidizing PUFAs and prostaglandins. However, the reaction provided involves the conversion of 4-androstene-3,17-dione to estrone, which is a different biochemical pathway related to steroid metabolism, not the metabolism of PUFAs or prostaglandins catalyzed by the gene product described. Thus, the gene and the provided reaction are not related. No
ENSG00000186377 Q8N118 MAR07940: 4-androstene-3,17-dione + NADPH + 2.0 O2 --> estrone + formate + 2.0 H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that specifically catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, but has no hydroxylase activity toward various fatty acids, steroids, and prostaglandins. The provided reaction involves the conversion of 4-androstene-3,17-dione to estrone, which is not a substrate for the cytochrome P450 monooxygenase encoded by this gene. Therefore, the gene and the reaction are unrelated. No
ENSG00000108242 P33260 MAR07940: 4-androstene-3,17-dione + NADPH + 2.0 O2 --> estrone + formate + 2.0 H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene in question, ENSG00000108242, encodes a cytochrome P450 monooxygenase which is involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid to 4-hydroxyretinoate. However, the provided reaction involves the conversion of 4-androstene-3,17-dione to estrone, which is not related to retinoid metabolism or the specific enzymatic activity of the gene described. Hence, the gene and the reaction are unrelated. No
ENSG00000186377 Q8N118 MAR07941: NADPH + 2.0 O2 + testosterone --> estradiol-17beta + formate + 2.0 H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that catalyzes the epoxidation of the arachidonoyl moiety of anandamide, using molecular oxygen and NADPH via cytochrome P450 reductase. This gene does not have hydroxylase activity toward fatty acids, steroids, and prostaglandins. The provided reaction involves the conversion of testosterone to estradiol-17beta, which is a different process and involves different substrates and products. Therefore, the function and catalytic activity of the gene do not align with the reaction provided. No
ENSG00000138109 P11712 MAR07941: NADPH + 2.0 O2 + testosterone --> estradiol-17beta + formate + 2.0 H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000138109 encodes for a cytochrome P450 monooxygenase, which metabolizes various endogenous substrates such as fatty acids and steroids. It catalyzes reactions involving the insertion of an oxygen atom into substrates and the reduction of the second oxygen atom into a water molecule, using electrons from NADPH via cytochrome P450 reductase. The gene's documented catalytic activities include epoxidation of polyunsaturated fatty acids, hydroxylation of carbon-hydrogen bonds, metabolism of cholesterol into 25-hydroxycholesterol, formation of catechol estrogens, hydroxylation of fatty acids and metabolites of plant monoterpenes, and metabolic activities related to drugs like S-warfarin and diclofenac. The provided reaction, which converts testosterone to estradiol-17beta, formate, and water, does not align with the described functions and substrate specificities of this gene, indicating that it is not relevant to the reaction. No
ENSG00000186377 Q8N118 MAR07957: H+ + NADPH + O2 + testosterone --> 19-hydroxytestosterone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that specifically catalyzes the epoxidation of anandamide and does not exhibit hydroxylase activity towards fatty acids, steroids, and prostaglandins. The reaction described involves the conversion of testosterone to 19-hydroxytestosterone, a process that the enzyme encoded by this gene does not participate in, as its catalytic activity is specific to the epoxidation of anandamide and not the hydroxylation of testosterone. No
ENSG00000186160 Q86W10 MAR07957: H+ + NADPH + O2 + testosterone --> 19-hydroxytestosterone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186160 encodes a cytochrome P450 monooxygenase that primarily catalyzes the in-chain oxidation of fatty acids, hydroxylates lauric and myristic acids, and catalyzes the epoxidation of double bonds of polyunsaturated fatty acids, using NADPH via cytochrome P450 reductase. The annotated catalytic activities include hydroxylation of fatty acids like dodecanoate and tetradecanoate and the epoxidation of arachidonic acid. However, the provided reaction involves the hydroxylation of testosterone to produce 19-hydroxytestosterone, which is not listed among the catalyzed reactions for the referenced gene. Therefore, there is no direct support connecting the gene's function to this specific reaction involving testosterone. No
ENSG00000108242 P33260 MAR07957: H+ + NADPH + O2 + testosterone --> 19-hydroxytestosterone + H2O + NADP+ ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974 Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000108242 codes for a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid (atRA) to 4-hydroxyretinoate. This process involves molecular oxygen and NADPH. However, the reaction provided involves the conversion of testosterone to 19-hydroxytestosterone, which is unrelated to the functionality of the cytochrome P450 monooxygenase described for ENSG00000108242. No
feiranl commented 1 month ago

@edkerk Ed could you also check this one?

edkerk commented 1 month ago

Many of the reactions involve cytochrome P450s, which can often can take quite a few different substrates, so that a very thorough curation would be required to further clean this up. Also there seems to be some reactions localized to the cytosol while others are in ER.

For a few reactions it was not too difficult to resolve what should be the new gene associations:

Reaction New grRule Pubmed ID Comment
MAR01927 ENSG00000140459 or ENSG00000138061 39395524  
MAR02029 ENSG00000137869 34095690 Should maybe be [r] only, but included as MAR07940
MAR02030 ENSG00000137869 34095690 Should maybe be [r] only, but included as MAR07940
MAR02031 ENSG00000137869 34095690 Should maybe be [r] only, but included as MAR07940
MAR02032 ENSG00000137869 34095690 Should maybe be [r] only, but included as MAR07941
MAR02033 ENSG00000137869 34095690 Should maybe be [r] only, but included as MAR07941
MAR02034 ENSG00000137869 34095690 Should maybe be [r] only, but included as MAR07941
MAR07940 ENSG00000137869 34095690  
MAR07941 ENSG00000137869 34095690  
MAR07957 ENSG00000137869 34095690  
MAR02038 ENSG00000101846 37951289  

Related to that, I identified the following curations:

Gene Comment Pubmed ID
ENSG00000137869 Should not be linked to any other reactions besides the ones above 34095690
ENSG00000101846 All reactions that this gene is associated to should be irreversible towards the direction that produces sulfate 37951289

The other reactions were too complex to fully resolve, but at least the following genes can be removed:

Reaction To remove
MAR02042 ENSG00000155016, ENSG00000186377, ENSG00000186526
MAR02043 ENSG00000108242, ENSG00000134716, ENSG00000186377
MAR02053 ENSG00000186377, ENSG00000186160, ENSG00000186204, ENSG00000108242
MAR02054 ENSG00000186204, ENSG00000155016, ENSG00000186377, ENSG00000186526
MAR02077 ENSG00000115705
MAR02089 ENSG00000115705

Those are all the curations I would suggest for this Issue.