GPR curation for Leukotriene metabolism (4)

[JHL-452b]

Current behavior:

19 genes are discovered to be uncorrelated with their corresponding reactions in Leukotriene metabolism.

Gene	UniProtID	Reaction	GPR	Correlation	Manually check
ENSG00000204160	Q9NUE0	MAR01088: CoA + H+ + N-acetyl-LTE4 <=> acetyl-CoA + leukotriene E4	ENSG00000023041 or ENSG00000054392 or ENSG00000087253 or ENSG00000090971 or ENSG00000097033 or ENSG00000099904 or ENSG00000100344 or ENSG00000102030 or ENSG00000102312 or ENSG00000102383 or ENSG00000103066 or ENSG00000104219 or ENSG00000106636 or ENSG00000109065 or ENSG00000110583 or ENSG00000111684 or ENSG00000121579 or ENSG00000122390 or ENSG00000123684 or ENSG00000125505 or ENSG00000133328 or ENSG00000135372 or ENSG00000136247 or ENSG00000141446 or ENSG00000143797 or ENSG00000144035 or ENSG00000153395 or ENSG00000153786 or ENSG00000156599 or ENSG00000159714 or ENSG00000160446 or ENSG00000163812 or ENSG00000163958 or ENSG00000167011 or ENSG00000171307 or ENSG00000171320 or ENSG00000172197 or ENSG00000172954 or ENSG00000173418 or ENSG00000174165 or ENSG00000175048 or ENSG00000175893 or ENSG00000176454 or ENSG00000177054 or ENSG00000177108 or ENSG00000177669 or ENSG00000180776 or ENSG00000184210 or ENSG00000184307 or ENSG00000184788 or ENSG00000186908 or ENSG00000188706 or ENSG00000188818 or ENSG00000203972 or ENSG00000204160 or ENSG00000206077 or ENSG00000243477	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000204160 encodes a palmitoyltransferase that facilitates the addition of palmitate to protein substrates, and its catalytic activity involves the reaction of hexadecanoyl-CoA with L-cysteinyl-[protein] to form CoA and S-hexadecanoyl-L-cysteinyl-[protein]. In contrast, the provided reaction involves CoA, H+, N-acetyl-LTE4, acetyl-CoA, and leukotriene E4, which is not related to the palmitoylation process carried out by the gene¡¯s product. Therefore, the gene and the reaction are not related based on the provided annotation.	No
ENSG00000143797	Q6ZWT7	MAR01088: CoA + H+ + N-acetyl-LTE4 <=> acetyl-CoA + leukotriene E4	ENSG00000023041 or ENSG00000054392 or ENSG00000087253 or ENSG00000090971 or ENSG00000097033 or ENSG00000099904 or ENSG00000100344 or ENSG00000102030 or ENSG00000102312 or ENSG00000102383 or ENSG00000103066 or ENSG00000104219 or ENSG00000106636 or ENSG00000109065 or ENSG00000110583 or ENSG00000111684 or ENSG00000121579 or ENSG00000122390 or ENSG00000123684 or ENSG00000125505 or ENSG00000133328 or ENSG00000135372 or ENSG00000136247 or ENSG00000141446 or ENSG00000143797 or ENSG00000144035 or ENSG00000153395 or ENSG00000153786 or ENSG00000156599 or ENSG00000159714 or ENSG00000160446 or ENSG00000163812 or ENSG00000163958 or ENSG00000167011 or ENSG00000171307 or ENSG00000171320 or ENSG00000172197 or ENSG00000172954 or ENSG00000173418 or ENSG00000174165 or ENSG00000175048 or ENSG00000175893 or ENSG00000176454 or ENSG00000177054 or ENSG00000177108 or ENSG00000177669 or ENSG00000180776 or ENSG00000184210 or ENSG00000184307 or ENSG00000184788 or ENSG00000186908 or ENSG00000188706 or ENSG00000188818 or ENSG00000203972 or ENSG00000204160 or ENSG00000206077 or ENSG00000243477	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000143797 encodes an acyltransferase involved in the reacylation of lysophospholipids and the phospholipid remodeling pathway, catalyzing reactions that typically involve acyl-CoA and lysophospholipid substrates to produce phospholipids and CoA as products. The mention of various catalytic activities supports its role in these specific biochemical pathways. The provided reaction, however, describes a different process: the interconversion between CoA, H+, N-acetyl-LTE4, acetyl-CoA, and leukotriene E4. This reaction involves leukotrienes and is not aligned with the biological functions and catalytic activities of the gene product described. Therefore, there is no connection between the gene ENSG00000143797 and the provided reaction.	No
ENSG00000102312	Q9H237	MAR01088: CoA + H+ + N-acetyl-LTE4 <=> acetyl-CoA + leukotriene E4	ENSG00000023041 or ENSG00000054392 or ENSG00000087253 or ENSG00000090971 or ENSG00000097033 or ENSG00000099904 or ENSG00000100344 or ENSG00000102030 or ENSG00000102312 or ENSG00000102383 or ENSG00000103066 or ENSG00000104219 or ENSG00000106636 or ENSG00000109065 or ENSG00000110583 or ENSG00000111684 or ENSG00000121579 or ENSG00000122390 or ENSG00000123684 or ENSG00000125505 or ENSG00000133328 or ENSG00000135372 or ENSG00000136247 or ENSG00000141446 or ENSG00000143797 or ENSG00000144035 or ENSG00000153395 or ENSG00000153786 or ENSG00000156599 or ENSG00000159714 or ENSG00000160446 or ENSG00000163812 or ENSG00000163958 or ENSG00000167011 or ENSG00000171307 or ENSG00000171320 or ENSG00000172197 or ENSG00000172954 or ENSG00000173418 or ENSG00000174165 or ENSG00000175048 or ENSG00000175893 or ENSG00000176454 or ENSG00000177054 or ENSG00000177108 or ENSG00000177669 or ENSG00000180776 or ENSG00000184210 or ENSG00000184307 or ENSG00000184788 or ENSG00000186908 or ENSG00000188706 or ENSG00000188818 or ENSG00000203972 or ENSG00000204160 or ENSG00000206077 or ENSG00000243477	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000102312 encodes a protein-serine O-palmitoleoyltransferase involved in the Wnt signaling pathway by attaching palmitoleate to Wnt proteins. The reaction associated with this gene catalyzes the transfer of palmitoleoyl groups to serine residues on Wnt proteins. However, the provided reaction involves the conversion of N-acetyl-LTE4 to leukotriene E4 and acetyl-CoA, a process unrelated to Wnt protein modification or the Wnt signaling pathway regulated by the gene. Therefore, there is no direct connection between the gene function and the provided reaction.	No
ENSG00000180776	Q5W0Z9	MAR01088: CoA + H+ + N-acetyl-LTE4 <=> acetyl-CoA + leukotriene E4	ENSG00000023041 or ENSG00000054392 or ENSG00000087253 or ENSG00000090971 or ENSG00000097033 or ENSG00000099904 or ENSG00000100344 or ENSG00000102030 or ENSG00000102312 or ENSG00000102383 or ENSG00000103066 or ENSG00000104219 or ENSG00000106636 or ENSG00000109065 or ENSG00000110583 or ENSG00000111684 or ENSG00000121579 or ENSG00000122390 or ENSG00000123684 or ENSG00000125505 or ENSG00000133328 or ENSG00000135372 or ENSG00000136247 or ENSG00000141446 or ENSG00000143797 or ENSG00000144035 or ENSG00000153395 or ENSG00000153786 or ENSG00000156599 or ENSG00000159714 or ENSG00000160446 or ENSG00000163812 or ENSG00000163958 or ENSG00000167011 or ENSG00000171307 or ENSG00000171320 or ENSG00000172197 or ENSG00000172954 or ENSG00000173418 or ENSG00000174165 or ENSG00000175048 or ENSG00000175893 or ENSG00000176454 or ENSG00000177054 or ENSG00000177108 or ENSG00000177669 or ENSG00000180776 or ENSG00000184210 or ENSG00000184307 or ENSG00000184788 or ENSG00000186908 or ENSG00000188706 or ENSG00000188818 or ENSG00000203972 or ENSG00000204160 or ENSG00000206077 or ENSG00000243477	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000180776 encodes a palmitoyltransferase enzyme responsible for catalyzing the addition of palmitate to proteins, particularly cysteine residues, using various acyl-CoA substrates (such as hexadecanoyl-CoA, tetradecanoyl-CoA, and octadecanoyl-CoA). The described reactions involve the palmitoylation of proteins, a process key for protein localization and function. In contrast, the reaction provided involves the conversion of N-acetyl-LTE4 to leukotriene E4 (LTE4) with the concurrent production of acetyl-CoA. This reaction is unrelated to protein palmitoylation and involves different substrates and enzymes, indicating that the gene ENSG00000180776 does not participate in or affect this specific biochemical reaction.	No
ENSG00000173418	P61599	MAR01091: CoA + H+ + N-acetyl-LTE4 <=> acetyl-CoA + leukotriene E4	ENSG00000023041 or ENSG00000054392 or ENSG00000087253 or ENSG00000090971 or ENSG00000097033 or ENSG00000099904 or ENSG00000100344 or ENSG00000102030 or ENSG00000102312 or ENSG00000102383 or ENSG00000103066 or ENSG00000104219 or ENSG00000106636 or ENSG00000109065 or ENSG00000110583 or ENSG00000111684 or ENSG00000121579 or ENSG00000122390 or ENSG00000123684 or ENSG00000125505 or ENSG00000133328 or ENSG00000135372 or ENSG00000136247 or ENSG00000141446 or ENSG00000143797 or ENSG00000144035 or ENSG00000153395 or ENSG00000153786 or ENSG00000156599 or ENSG00000159714 or ENSG00000160446 or ENSG00000163812 or ENSG00000163958 or ENSG00000167011 or ENSG00000171307 or ENSG00000171320 or ENSG00000172197 or ENSG00000172954 or ENSG00000173418 or ENSG00000174165 or ENSG00000175048 or ENSG00000175893 or ENSG00000176454 or ENSG00000177054 or ENSG00000177108 or ENSG00000177669 or ENSG00000180776 or ENSG00000184210 or ENSG00000184307 or ENSG00000184788 or ENSG00000186908 or ENSG00000188706 or ENSG00000188818 or ENSG00000203972 or ENSG00000204160 or ENSG00000206077 or ENSG00000243477	Pending No, the gene is not relevant to the reaction provided. The provided gene, ENSG00000173418, encodes the catalytic subunit of the NatB complex, which is responsible for the acetylation of N-terminal methionine residues in specific peptides. This enzyme catalyzes the acetylation of N-terminal methionine combined with aspartic acid, glutamic acid, asparagine, or glutamine. However, the reaction provided involves the interconversion of CoA, H+, N-acetyl-LTE4, acetyl-CoA, and leukotriene E4, which is not related to the substrate specificity or function of the NatB complex. Thus, the gene is not relevant to the given reaction.	No
ENSG00000176454	Q643R3	MAR01091: CoA + H+ + N-acetyl-LTE4 <=> acetyl-CoA + leukotriene E4	ENSG00000023041 or ENSG00000054392 or ENSG00000087253 or ENSG00000090971 or ENSG00000097033 or ENSG00000099904 or ENSG00000100344 or ENSG00000102030 or ENSG00000102312 or ENSG00000102383 or ENSG00000103066 or ENSG00000104219 or ENSG00000106636 or ENSG00000109065 or ENSG00000110583 or ENSG00000111684 or ENSG00000121579 or ENSG00000122390 or ENSG00000123684 or ENSG00000125505 or ENSG00000133328 or ENSG00000135372 or ENSG00000136247 or ENSG00000141446 or ENSG00000143797 or ENSG00000144035 or ENSG00000153395 or ENSG00000153786 or ENSG00000156599 or ENSG00000159714 or ENSG00000160446 or ENSG00000163812 or ENSG00000163958 or ENSG00000167011 or ENSG00000171307 or ENSG00000171320 or ENSG00000172197 or ENSG00000172954 or ENSG00000173418 or ENSG00000174165 or ENSG00000175048 or ENSG00000175893 or ENSG00000176454 or ENSG00000177054 or ENSG00000177108 or ENSG00000177669 or ENSG00000180776 or ENSG00000184210 or ENSG00000184307 or ENSG00000184788 or ENSG00000186908 or ENSG00000188706 or ENSG00000188818 or ENSG00000203972 or ENSG00000204160 or ENSG00000206077 or ENSG00000243477	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000176454 encodes for an enzyme that displays acyl-CoA-dependent lysophospholipid acyltransferase activity, specifically targeting substrates such as lysophosphatidylethanolamine, lysophosphatidylcholine, and related compounds. The enzyme catalyzes reactions involving the addition of acyl groups to these substrates. In contrast, the reaction provided involves the conversion of N-acetyl-LTE4 to leukotriene E4 involving CoA and H+, which does not align with the specific activities or substrate specificity of the enzyme encoded by ENSG00000176454. Therefore, the gene is not relevant to the provided reaction.	No
ENSG00000125505	Q96N66	MAR01091: CoA + H+ + N-acetyl-LTE4 <=> acetyl-CoA + leukotriene E4	ENSG00000023041 or ENSG00000054392 or ENSG00000087253 or ENSG00000090971 or ENSG00000097033 or ENSG00000099904 or ENSG00000100344 or ENSG00000102030 or ENSG00000102312 or ENSG00000102383 or ENSG00000103066 or ENSG00000104219 or ENSG00000106636 or ENSG00000109065 or ENSG00000110583 or ENSG00000111684 or ENSG00000121579 or ENSG00000122390 or ENSG00000123684 or ENSG00000125505 or ENSG00000133328 or ENSG00000135372 or ENSG00000136247 or ENSG00000141446 or ENSG00000143797 or ENSG00000144035 or ENSG00000153395 or ENSG00000153786 or ENSG00000156599 or ENSG00000159714 or ENSG00000160446 or ENSG00000163812 or ENSG00000163958 or ENSG00000167011 or ENSG00000171307 or ENSG00000171320 or ENSG00000172197 or ENSG00000172954 or ENSG00000173418 or ENSG00000174165 or ENSG00000175048 or ENSG00000175893 or ENSG00000176454 or ENSG00000177054 or ENSG00000177108 or ENSG00000177669 or ENSG00000180776 or ENSG00000184210 or ENSG00000184307 or ENSG00000184788 or ENSG00000186908 or ENSG00000188706 or ENSG00000188818 or ENSG00000203972 or ENSG00000204160 or ENSG00000206077 or ENSG00000243477	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000125505 encodes an acyltransferase involved in the reacylation of lysophosphatidylinositol (LPI) to phosphatidylinositol (PI) as part of the Lands cycle and prefers arachidonoyl-CoA as an acyl donor. The catalytic activities described include various acyl-CoA and lysophosphatidylinositol or phosphocholine substrates, none of which correspond to the reaction involving CoA, H+, N-acetyl-LTE4, acetyl-CoA, and leukotriene E4. Thus, the annotated function and catalytic activities of the gene do not match the provided reaction.	No
ENSG00000160439	Q8NBN7	MAR01098: 13,14-dihydro-15-oxo-lipoxin A4 + NADP+ <=> 15-oxo-lipoxin A4 + H+ + NADPH	ENSG00000087076 or ENSG00000099377 or ENSG00000100867 or ENSG00000109854 or ENSG00000121039 or ENSG00000138400 or ENSG00000139988 or ENSG00000145439 or ENSG00000159692 or ENSG00000160439 or ENSG00000164039 or ENSG00000167733 or ENSG00000170426 or ENSG00000170786 or ENSG00000176387 or ENSG00000183921 or ENSG00000184860 or ENSG00000186153 or ENSG00000187134 or ENSG00000197894 or ENSG00000198074 or ENSG00000198189 or ENSG00000198610 or ENSG00000204228 or ENSG00000227471 or ENSG00000240857	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000160439 encodes a protein that primarily functions as a retinol dehydrogenase, which oxidizes all-trans-retinol and reduces all-trans-retinal with a clear preference for NADP and has no activity toward steroids. The catalytic activity involves the conversion of all-trans-retinol to all-trans-retinal. The reaction provided, however, involves the conversion of 13,14-dihydro-15-oxo-lipoxin A4 to 15-oxo-lipoxin A4, which does not involve retinoids and is not aligned with the gene's annotated function.	No
ENSG00000159228	P16152	MAR01106: leukotriene B4 + NAD+ --> 12-keto-LTB4 + H+ + NADH	ENSG00000157326 or ENSG00000159228 or ENSG00000159231	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000159228 encodes for an NADPH-dependent reductase that catalyzes a variety of reactions involving the reduction of carbonyl compounds, prostaglandins, antitumor anthracyclines, S-nitrosoglutathione, and glucocorticoids, utilizing NADPH as a cofactor. However, the reaction provided involves the oxidation of leukotriene B4 using NAD+ as a cofactor to produce 12-keto-LTB4, H+, and NADH, which does not match the substrate specificity or the type of cofactor used by the enzyme encoded by ENSG00000159228. Therefore, the gene does not participate in the given reaction.	No
ENSG00000147576	Q8IWW8	MAR01131: 20-OH-LTB4 + NAD+ <=> 20-oxo-LTB4 + H+ + NADH	ENSG00000147576 or ENSG00000172955 or ENSG00000187758 or ENSG00000196344 or ENSG00000196616 or ENSG00000197894 or ENSG00000198099 or ENSG00000248144	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000147576 encodes an enzyme that catalyzes the oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) and the reduction of 2-ketoglutarate to D-2-hydroxyglutarate (D-2-HG). The provided reaction involves the conversion of 20-OH-LTB4 to 20-oxo-LTB4 using NAD+ and producing NADH. Since the gene¡¯s function does not involve the substrates or products of the provided reaction (20-OH-LTB4, NAD+, 20-oxo-LTB4, H+), the gene is not relevant to this reaction.	No
ENSG00000100197	P10635	MAR01150: 5-oxo-6-trans-LTB4 + H+ + NADPH <=> 6,7-dihydro-5-oxo-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000100197 encodes a cytochrome P450 monooxygenase involved in the metabolism of fatty acids, steroids, retinoids, endocannabinoids, cholesterol, all-trans retinol, and oxidative metabolism of certain drugs. It catalyzes reactions involving molecular oxygen and NADPH, inserting oxygen atoms into various substrates. The provided reaction (5-oxo-6-trans-LTB4 + H+ + NADPH <=> 6,7-dihydro-5-oxo-LTB4 + NADP+) does not involve the cytochrome P450 monooxygenase's known substrates or reaction types and thus is unrelated to the gene's annotated functions.	No
ENSG00000197408	P20813	MAR01151: 5-oxo-6-trans-LTB4 + H+ + NADPH <=> 6,7-dihydro-5-oxo-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000197408 encodes for a cytochrome P450 monooxygenase, which is involved in the metabolism of endocannabinoids and steroids. Its roles include epoxidation of arachidonoylethanolamide, hydroxylation of steroid hormones, and oxidative metabolism of xenobiotics. The specified reaction involves the conversion of 5-oxo-6-trans-LTB4 to 6,7-dihydro-5-oxo-LTB4 using NADPH. This reaction does not involve the metabolic functions associated with the cytochrome P450 monooxygenase encoded by ENSG00000197408. Therefore, the gene is not relevant to the reaction provided.	No
ENSG00000186204	Q9HCS2	MAR01153: 6,7-dihydro-5-oxo-LTB4 + H+ + NADPH <=> 6,7-dihydro-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186204 encodes a cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFAs) and certain xenobiotics, catalyzing hydroxylation and epoxidation reactions. The provided reaction, which involves the conversion of 6,7-dihydro-5-oxo-LTB4 to 6,7-dihydro-LTB4 using NADPH, does not fall within the known substrates or reaction types catalyzed by this cytochrome P450, as outlined in the annotations. Thus, the gene is not relevant to this specific reaction.	No
ENSG00000186204	Q9HCS2	MAR01154: 6,7-dihydro-5-oxo-LTB4 + H+ + NADPH <=> 6,7-dihydro-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186204 encodes for a cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs) and the metabolism of xenobiotics. Its catalytic activities include the hydroxylation and epoxidation of PUFAs such as eicosatetraenoic, docosapentaenoic, and docosahexaenoic acids. However, the provided reaction involves the conversion of 6,7-dihydro-5-oxo-LTB4 to 6,7-dihydro-LTB4, which is not related to the catalytic activities or substrate specificities of the cytochrome P450 monooxygenase encoded by this gene.	No
ENSG00000186204	Q9HCS2	MAR01155: 6,7-dihydro-5-oxo-LTB4 + H+ + NADPH <=> 6,7-dihydro-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186204 encodes a cytochrome P450 monooxygenase that primarily metabolizes endogenous polyunsaturated fatty acids (PUFAs) through various hydroxylation and epoxidation reactions, as detailed in the provided annotation. The reaction provided involves the conversion of 6,7-dihydro-5-oxo-LTB4 to 6,7-dihydro-LTB4 using NADPH, which is distinct from the P450-mediated reactions and substrates described for this gene, indicating no direct relevance.	No
ENSG00000186526	P98187	MAR01155: 6,7-dihydro-5-oxo-LTB4 + H+ + NADPH <=> 6,7-dihydro-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186526 codes for a cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids and their derivatives. It catalyzes hydroxylation and epoxidation reactions using molecular oxygen and NADPH. The provided reaction involves the conversion of 6,7-dihydro-5-oxo-LTB4 to 6,7-dihydro-LTB4 using NADPH, which is not among the substrates or reactions catalyzed by the gene product according to the given annotation. Therefore, the gene and the reaction are not related.	No
ENSG00000186526	P98187	MAR01156: 6,7-dihydro-5-oxo-LTB4 + H+ + NADPH <=> 6,7-dihydro-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186526 encodes a cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids and their derivatives by catalyzing various hydroxylation and epoxidation reactions. All the described catalytic activities involve the insertion of one oxygen atom into the substrate using molecular oxygen and NADPH, reducing the second oxygen into a water molecule. In contrast, the provided reaction involves the reduction of 6,7-dihydro-5-oxo-LTB4 to 6,7-dihydro-LTB4 using NADPH, which does not align with the oxidation and hydroxylation activities performed by the cytochrome P450 monooxygenase. Therefore, the gene and the reaction are not related.	No
ENSG00000186526	P98187	MAR01196: 5-oxo-(6E)-12-epi-LTB4 + H+ + NADPH <=> 6,7-dihydro-5-oxo-12-epi-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186526 encodes a cytochrome P450 monooxygenase involved in the metabolism of polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives, including hydroxylation and epoxidation reactions. The specific reactions catalyzed by this gene involve substrates like eicosatetraenoate, prostaglandins, docosahexaenoate, and docosapentaenoate. However, the provided reaction involves the reduction of 5-oxo-(6E)-12-epi-LTB4 to 6,7-dihydro-5-oxo-12-epi-LTB4 using NADPH, which does not match the types of reactions or substrates associated with the gene's known functions. Therefore, the gene's annotated enzymatic activities do not relate to the provided reaction.	No
ENSG00000161533	Q15067	MAR01205: 5-oxo-12(S)-hydroxy-eicosa-(8E,10E,14Z)-trienoyl-CoA + FAD --> 5-oxo-12(S)-hydroxy-eicosa-(2E,8E,10E,14Z)-tetraenoyl-CoA + FADH2	ENSG00000087008 or ENSG00000161533	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The provided gene (ENSG00000161533) is primarily involved in the catalysis of the desaturation of fatty acyl-CoAs to 2-trans-enoyl-CoAs, producing hydrogen peroxide (H2O2) in the process, as indicated repeatedly across various reactions it catalyzes. However, the provided reaction involves the conversion of 5-oxo-12(S)-hydroxy-eicosa-(8E,10E,14Z)-trienoyl-CoA to 5-oxo-12(S)-hydroxy-eicosa-(2E,8E,10E,14Z)-tetraenoyl-CoA with the involvement of FAD and FADH2, which is distinct in terms of substrates and cofactors used. The gene's annotated functions do not include this specific reaction, therefore, it is not relevant.	No

[JHL-452b]

For Leukotriene metabolism, I continued to add 3 unrelated gene-reaction pairs discoverd by GPT.

Gene	UniProtID	Reaction	GPR	Correlation	Check
ENSG00000203972	Q5SZD4	MAR01088: CoA + H+ + N-acetyl-LTE4 <=> acetyl-CoA + leukotriene E4	ENSG00000023041 or ENSG00000054392 or ENSG00000087253 or ENSG00000090971 or ENSG00000097033 or ENSG00000099904 or ENSG00000100344 or ENSG00000102030 or ENSG00000102312 or ENSG00000102383 or ENSG00000103066 or ENSG00000104219 or ENSG00000106636 or ENSG00000109065 or ENSG00000110583 or ENSG00000111684 or ENSG00000121579 or ENSG00000122390 or ENSG00000123684 or ENSG00000125505 or ENSG00000133328 or ENSG00000135372 or ENSG00000136247 or ENSG00000141446 or ENSG00000143797 or ENSG00000144035 or ENSG00000153395 or ENSG00000153786 or ENSG00000156599 or ENSG00000159714 or ENSG00000160446 or ENSG00000163812 or ENSG00000163958 or ENSG00000167011 or ENSG00000171307 or ENSG00000171320 or ENSG00000172197 or ENSG00000172954 or ENSG00000173418 or ENSG00000174165 or ENSG00000175048 or ENSG00000175893 or ENSG00000176454 or ENSG00000177054 or ENSG00000177108 or ENSG00000177669 or ENSG00000180776 or ENSG00000184210 or ENSG00000184307 or ENSG00000184788 or ENSG00000186908 or ENSG00000188706 or ENSG00000188818 or ENSG00000203972 or ENSG00000204160 or ENSG00000206077 or ENSG00000243477	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000203972 encodes a protein that catalyzes the conjugation of long-chain fatty acyl-CoA thioesters and glycine to produce long-chain N-(fatty acyl)glycine, which is an intermediate in the primary fatty acid amide biosynthetic pathway. The provided reaction, however, involves the conversion of CoA, H+, and N-acetyl-LTE4 to acetyl-CoA and leukotriene E4, which does not appear to involve long-chain fatty acyl-CoA thioesters or glycine, nor does it fit within the described functional activity of the gene product. The reaction also does not align with the specific catalytic activities (e.g., producing N-(9Z-octadecenoyl)glycine or N-hexadecanoylglycine) detailed for this gene. Hence, the gene is not relevant to the reaction provided.	No
ENSG00000159228	P16152	MAR01101: leukotriene B4 + NAD+ --> 12-keto-LTB4 + H+ + NADH	ENSG00000157326 or ENSG00000159228 or ENSG00000159231	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000159228 encodes an NADPH-dependent reductase that catalyzes the reduction of various carbonyl compounds including quinones, prostaglandins, menadione, xenobiotics, and antitumor anthracyclines. It is involved in cortisol/corticosterone metabolism and S-nitrosoglutathione reduction. However, the provided reaction involves the oxidation of leukotriene B4 using NAD+, resulting in 12-keto-LTB4, H+, and NADH. This reaction does not match the catalytic activities of the enzyme encoded by the gene, which predominantly uses NADP+/NADPH as coenzymes. Therefore, the gene is not relevant to the reaction provided.	No
ENSG00000138109	P11712	MAR01196: 5-oxo-(6E)-12-epi-LTB4 + H+ + NADPH <=> 6,7-dihydro-5-oxo-12-epi-LTB4 + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000138109 encodes a cytochrome P450 monooxygenase involved in various metabolic processes, including the metabolism of fatty acids, steroids, cholesterol, and drugs by inserting oxygen atoms into substrates. It is associated with reactions involving the epoxidation of polyunsaturated fatty acids, hydroxylation of carbon-hydrogen bonds, and metabolism of cholesterol to 25-hydroxycholesterol, among others. However, the given reaction involves the conversion of 5-oxo-(6E)-12-epi-LTB4 to 6,7-dihydro-5-oxo-12-epi-LTB4 using NADPH, which does not involve the monooxygenase function or any of the described catalytic activities of the gene ENSG00000138109. Therefore, the gene and the reaction are not related.	No

[feiranl]

LGTM!