SziKayLeung / Whole_Transcriptome_Paper

17 stars 3 forks source link

Convert the SRA file into BAM files #4

Open Kratos12138 opened 8 months ago

Kratos12138 commented 8 months ago

Hi, Leung,

I'm encountering an issue converting SRA files into BAM files with headers for subsequent analysis.

I've been using the following command to convert the raw SRA files into subreads BAM files:

sam-dump -u -r SRR12660769.sra | samtools view -bS - > SRR12660769.bam

I have two questions:

First, I need information about the file format of your uploaded files. Second, could you provide instructions on how to convert SRA files to operational BAM files? Alternatively, is there another source where I can download subreads.ccs.bam files? Thanks a lot.

MenglinC commented 7 months ago

Hi,I have the same question!Do you have any idea now?