Essential Oil P Study 2022 SOP

Project Overview

To Do: Review checklist

Project Objectives - To determine EO hemp (Cannabis sativa) response to applied phosphorus. Project Description - Essential oil P Study Project Protocol - 1 varieties x 5 rates x 4 reps. (5 trt). Field size: L 118' x W 84’. (0.23 ac). Plot size: L 25' x W 12'. (300 sqft). Field Design - RCB with 4 reps. Blocked by replication. Field Diagrams - Maps can be found on TEAMS. Start Date- 5/26-27. Projected End Date- September/October. Project Manager(s)- Daniel, Ajit. Planting Date- 8/2/2022. Crop(s)- Hemp: Essential oil - cv. Wife (CBD). Irrigation - Drip tape. Applications - Six (6) Nitrogen treatments, split between **four (4) applications. Planting methods - (Wife)- 3' Mounds, planted in center 1.25'. Transplanted by hand.

(Maverick)- 3' Mounds, planted offset from center. Seeded by (new seeder). Data Collection & Sampling - Project Maintenance - Ajit, Daniel. Equipment needed - Moldboard plow, Harrow disk, raised bed shaper, roto-tiller, herbicide wagon, others - TBD. Field crew assistance - Yes. For irrigation and land preparation. _
Land Request

Preferred block- Block 10. Section- Eastside. Area/Acres requested- L 118' x W 84’. (0.23 ac). Greenhouse - Yes. To Do: Update checklist -[] Complete TREC Land Request -[] Complete TREC Greenhouse Request -[] Request faculty sponsor to complete UFarm request.

Land Preparation

Cultivation (mowing, moldboard plow, disc and level the field, form raised beds, drilling holes, etc.) - Row Length - 25 ft. In-Row Spacing - 8.5 inches for direct seed. 6 plants for transplants. Between-Row Spacing - 6 ft. Field Buffers - 30 ft. Seeding? (Direct, Broadcast, Incorporated, Drill, Earthway, etc.) - direct. Transplants? - Yes. Support Structure (Rebar, String, etc.) - Yes, bamboo for transplants. Plastic Mulch? - Biodegradeable. Full image of field layout, design, and plot arrangement - Maps and layouts can be found on TEAMS. Date to complete land preparation - 5/25-26. _ To Do: Update checklist -[] Complete TREC field preparation request -[] Row Crop -[] Plastic Culture

Irrigation

Fertigation? Yes. Drip Tape? (drip placement, emitter spacing, flow-rate GPM/100 ft., etc.) - Drip tape. 2 lines per bed? Solid set? (overhead irrigation, micro-sprinkler) - Solid set - overhead. Irrigation Timing (frequency and trigger) - As needed *[Once per week for the first 2-3 weeks to germinate and establish**]. Irrigation Amount (per application) - 1 inch. To Do: Update checklist -[] Complete TREC fieldwork request for irrigation.

Fertilizer Application

Label Name - Urea (N), Triple Super Phosphate (P), Sulfate of Potash (K) Analysis rates (NPK, etc.) - 46-0-0 (N), 0-46-0 (P), 0-0-50 (K) Application rate per acre -

Urea (N) 0 lbs N/ac 50 lbs N/ac 100 lbs N/ac 150 lbs N/ac 200 lbs N/ac 250 lbs N/ac. TSP
Triple Super Phosphate (P) 60 lbs P2O5/ac
Sulfate of Potash (K) 300 lbs K2O/ac

Application method (banded on top of the bed, soil incorporation, etc.) - Pre-plant incorporated - except nitrogen. Application timing and frequency - TBD. Lab or field personnel - Ajit, Daniel. To Do: Update checklist -[] Fertilizer request form

Herbicide Application

Label Name - Dual Magnum. Active ingredient(s) and concentration - S-metolachlor, benoxacor. Resistance Management: Group 15 Herbicide. Restricted Entry Interval (REI): 24 hours. Application rate per acre - 1 pint. Application method (pre-plant broadcast incorporated, shank or drip applied, etc.) - pre-planting, sprayer. Application timing and frequency - Once at pre-planting. Lab or field personnel - Daniel, Ajit, Jacob. To Do: Update checklist -[] Worker Protection Standard Training -[] Review "Training Workers and Handlers under the 2016 Revision Requirements" by Frederick M. Fishel. -[] Found in the TREC network Unit drive > TREC Pesticide Use information > WPS information -[] Pesticide/Herbicde request form

Project Maintenance Events

Research treatment application - Six (6) Nitrogen treatments, split between four (4) applications. - TBD. Weeding - PRE: Dual Magnum. POST: cultivation or hooded applications.

POST: Roto-tilling buffers monthly(as needed). Hooded herbicide application for furrows and between beds. Scouting - Daily: Drip tape leaks, Tensiometer readings, plant health. Sampling - Harvesting and Sampling - Project removal - September/October. To Do: Update checklist -[] Equipment Request -[] Complete TREC fieldwork request well ahead of maintenance events.
Data Collection & Sampling

Database - TEAMS, GitHub. Data type: Rainfall, irrigation, temperature Soil moisture Mehlich 3 P, pre-plant, 0-6”, block-level Total N (dry combustion), pre-plant, 0-6”, block-level NO3- & NH4+, pre-plant, 0-6”, block-level SOM, pre-plant, 0-6”, block-level Soil water holding capacity Plants/m2 after emergence Leaf tissue nutrient concentrations (N & P) i. Stage 1 ii. Stage 2 iii. Stage 3 iv. Stage 4 v. Stage 5 Biomass at harvest Yield, moisture, test weight Mehlich 3 P, post-harvest, 0-6”, plot-level Total N (dry combustion), post-harvest, 0-6”, plot-level NO3- & NH4+, post-harvest, 0-6”, plot-level SOM, post-harvest, 0-6”, plot-level. Data storage - Metadata - Data collection - Protocols - Timing - Logistics - Sample processing and analysis - _

Contingencies

What important decisions are next to be made or remain a challenge?

Predicting weather patterns.
Making progress despite weather delays/ shifting efforts.

What could possibly go wrong?

Weather delays.
Changes to experiment, field design/ layout.
Equipment issues/ calibration delays.

TREC-Agroecology / hemp