Since I tried to build an index for 3' tagged-sequencing, I didn't add the --lasttwoexon parameter, and then the UnboundLocalError raised: UnboundLocalError: local variable 'TFseq' referenced before assignment
I checked the source code, and I guess the problem is when not using --lasttwoexon parameter, there is no TFseq variable assigned before the conditional statement (the variable used here is seq not TFseq, shown below).
I'll try modifying the variable locally to verify it, please correct me if I'm wrong.
Hi,
When I tried to use makeTFfast to build an index, I used the following command:
Since I tried to build an index for 3' tagged-sequencing, I didn't add the --lasttwoexon parameter, and then the UnboundLocalError raised:
UnboundLocalError: local variable 'TFseq' referenced before assignment
I checked the source code, and I guess the problem is when not using --lasttwoexon parameter, there is no TFseq variable assigned before the conditional statement (the variable used here is seq not TFseq, shown below).
I'll try modifying the variable locally to verify it, please correct me if I'm wrong.
Best, Qian