绘图问题

[wangzhaohui-001]

老师，你好！ 我有几个问题请教一下。

1. 我在运行 tcbf plot-syn-pair --output test --reference BH --chrom Chr05 --start 13000000 --end 13300000 --plot test.pdf 时候出现下面错误，我已经改过bound 和one_to_many Checking dependency! Traceback (most recent call last): File "/home/ug1561/.conda/envs/py39/bin/tcbf", line 7, in exec(compile(f.read(), file, 'exec')) File "/home/ug1561/workspace/wangzhaohui/biosoft/Tcbf-main/bin/tcbf", line 173, in tcbf() File "/home/ug1561/.conda/envs/py39/lib/python3.9/site-packages/click/core.py", line 1130, in call return self.main(args, kwargs) File "/home/ug1561/.conda/envs/py39/lib/python3.9/site-packages/click/core.py", line 1055, in main rv = self.invoke(ctx) File "/home/ug1561/.conda/envs/py39/lib/python3.9/site-packages/click/core.py", line 1657, in invoke return _process_result(sub_ctx.command.invoke(sub_ctx)) File "/home/ug1561/.conda/envs/py39/lib/python3.9/site-packages/click/core.py", line 1404, in invoke return ctx.invoke(self.callback, ctx.params) File "/home/ug1561/.conda/envs/py39/lib/python3.9/site-packages/click/core.py", line 760, in invoke return __callback(args, **kwargs) File "/home/ug1561/workspace/wangzhaohui/biosoft/Tcbf-main/bin/tcbf", line 158, in plot_syn_pair plot_synteny(output, reference, chrom, start, end, order, plot) File "/home/ug1561/workspace/wangzhaohui/biosoft/Tcbf-main/tcbf/plot_tad_structure.py", line 424, in plot_synteny fig = plot_main(workdir, reference, chrom, start, end, species_order) File "/home/ug1561/workspace/wangzhaohui/biosoft/Tcbf-main/tcbf/plot_tad_structure.py", line 415, in plot_main plot_boundary_pair(workdir, File "/home/ug1561/workspace/wangzhaohui/biosoft/Tcbf-main/tcbf/plot_tad_structure.py", line 343, in plot_boundary_pair tmp = data.loc[:, [reference, s]].dropna().apply(lambda x: x.str.strip()).applymap( File "/home/ug1561/.conda/envs/py39/lib/python3.9/site-packages/pandas/core/generic.py", line 5989, in getattr return object.getattribute(self, name) AttributeError: 'DataFrame' object has no attribute 'to_list' 2.根据TAD_groups_count将所有大于0的视为保守的，一般情况下这样的区域会在最终结果中占比多少？ 以上问题请老师给你帮，谢谢！