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Teichlab / cellphonedb

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Designation of clusters that express ligand vs. receptor #228

Closed 14lwt1 closed 3 years ago

14lwt1 commented 3 years ago

Hi,

I have a naïve question regarding the workflow of CellphoneDB. From the web portal, it is stated that "the mean of the average receptor expression level in a cluster and the average ligand expression level in the interacting cluster" is used for permutation. But how does the algorithm decide whether a cluster secretes ligands or expresses receptors? (https://www.cellphonedb.org/explore-sc-rna-seq) image Isn't it possible for a cluster to express both the ligand and receptor?

Thanks Henry

luzgaral commented 3 years ago

Hi Henry,

CellphoneDB test all possible combinations, therefore all clusters can express the ligand and/or the receptor. For example, when testing if cellA can communicate to cellB, cellphoneDB will test R1-cellA ::: L1-cellB and viceversa R1-cellB ::: L1-cellA.

Hope this makes sense.

Luz