Open singcell opened 3 years ago
Hi @singcell, sounds like either an error with either your meta file or with pandas. Could you please:
a) provide your version of CellPhoneDB and pandas? (pip show pandas cellphonedb
)
b) check that you're using the right separator for the file? (if it's .txt
then it should be \t
(tab)
Thank you for your reply. Please see below.
Name: CellPhoneDB Version: 2.1.7 Summary: Inferring cell-cell communication Home-page: https://cellphonedb.org Author: TeichLab Author-email: contact@cellphonedb.org License: MIT Location: /usr/local/lib/python3.7/site-packages Requires: Flask-Testing, anndata, geosketch, h5py, numpy, Flask-RESTful, click, PyYAML, rpy2, requests, pika, flask, SQLAlchemy, boto3, tqdm, pandas, scikit-learn Required-by:
b) I tried both and comma.
Hi @singcell thank you for checking the versions. I think the issue comes from the formatting of that file. Without having a closer look at your input files is hard to tell. Could you share your cellphonedb_meta.txt
?
I am trying to run CellphoneDB but I am getting error messages "single positional indexer is out-of-bounds" and "Error processing Meta data". please see the messages below and let me know how to fix thiss error.
cellphonedb method statistical_analysis cellphonedb_meta.txt cellphonedb_count.txt /usr/local/lib/python3.7/site-packages/sklearn/utils/deprecation.py:144: FutureWarning: The sklearn.cluster.kmeans module is deprecated in version 0.22 and will be removed in version 0.24. The corresponding classes / functions should instead be imported from sklearn.cluster. Anything that cannot be imported from sklearn.cluster is now part of the private API. warnings.warn(message, FutureWarning) [ ][APP][20/06/21-23:25:54][WARNING] Latest local available version is
v2.0.0
, using it [ ][APP][20/06/21-23:25:54][WARNING] User selected downloaded databasev2.0.0
is available, using it [ ][CORE][20/06/21-23:25:54][INFO] Initializing SqlAlchemy CellPhoneDB Core [ ][CORE][20/06/21-23:25:54][INFO] Using custom database at /Users/kate/.cpdb/releases/v2.0.0/cellphone.db [ ][APP][20/06/21-23:25:54][INFO] Launching Method cpdb_statistical_analysis_local_method_launcher [ ][APP][20/06/21-23:25:54][INFO] Launching Method _set_paths [ ][APP][20/06/21-23:25:54][INFO] Launching Method _load_meta_counts [ ][CORE][20/06/21-23:28:56][INFO] Launching Method cpdb_statistical_analysis_launcher [ ][APP][20/06/21-23:28:56][ERROR] Unexpected error Traceback (most recent call last): File "/usr/local/lib/python3.7/site-packages/cellphonedb/src/core/preprocessors/method_preprocessors.py", line 28, in meta_preprocessor meta = pd.DataFrame(data={'cell_type': meta_raw.iloc[:, 1]}) File "/usr/local/lib/python3.7/site-packages/pandas/core/indexing.py", line 873, in getitem return self._getitem_tuple(key) File "/usr/local/lib/python3.7/site-packages/pandas/core/indexing.py", line 1443, in _getitem_tuple self._has_valid_tuple(tup) File "/usr/local/lib/python3.7/site-packages/pandas/core/indexing.py", line 702, in _has_valid_tuple self._validate_key(k, i) File "/usr/local/lib/python3.7/site-packages/pandas/core/indexing.py", line 1352, in _validate_key self._validate_integer(key, axis) File "/usr/local/lib/python3.7/site-packages/pandas/core/indexing.py", line 1437, in _validate_integer raise IndexError("single positional indexer is out-of-bounds") IndexError: single positional indexer is out-of-boundsDuring handling of the above exception, another exception occurred:
Traceback (most recent call last): File "/usr/local/lib/python3.7/site-packages/cellphonedb/src/api_endpoints/terminal_api/method_terminal_api_endpoints/method_terminal_commands.py", line 144, in statistical_analysis subsampler, File "/usr/local/lib/python3.7/site-packages/cellphonedb/src/local_launchers/local_method_launcher.py", line 64, in cpdb_statistical_analysis_local_method_launcher subsampler File "/usr/local/lib/python3.7/site-packages/cellphonedb/src/core/methods/method_launcher.py", line 50, in cpdb_statistical_analysis_launcher meta = method_preprocessors.meta_preprocessor(raw_meta) File "/usr/local/lib/python3.7/site-packages/cellphonedb/src/core/preprocessors/method_preprocessors.py", line 35, in meta_preprocessor raise ProcessMetaException cellphonedb.src.core.exceptions.ProcessMetaException.ProcessMetaException: Error processing Meta data