BLAST query/options error: Germline annotation database human/human_TR_V could not be found in [internal_data] directory
Please refer to the BLAST+ user manual.**
My IGDATA variable is set as /home/kfir/ncbi-igblast-1.22.0/bin.
And oddly enough this command runs with no errors when I set '-s Mmus'. But obviously, the results are of no use because my data is human.
What can be done to fix this? Am I the first one to encounter this behavior?
Hello, I have ran the following command on my data:
tracer assemble -s Hsap -c /home/kfir/tracer/tracer.conf -p 2 /home/kfir/Pemphigus_TCR_Analysis/TAU260324/1/C2_exp78_S1_L001_R1_001.fastq.gz /home/kfir/Pemphigus_TCR_Analysis/TAU260324/1/C2_exp78_S1_L001_R2_001.fastq.gz cell /home/kfir/Pemphigus_TCR_Analysis/TAU260324/1/tracer_C2_exp78_S1_L001
And in the Igblastn step it returns the following error:
**##Running IgBLAST## CMD failed: /home/kfir/ncbi-igblast-1.22.0/bin/igblastn -germline_db_V /home/kfir/tracer/resources/Hsap/igblast_dbs/TCR_V.fa -germline_db_D /home/kfir/tracer/resources/Hsap/igblast_dbs/TCR_D.fa -germline_db_J /home/kfir/tracer/resources/Hsap/igblast_dbs/TCR_J.fa -domain_system imgt -organism human -ig_seqtype TCR -show_translation -num_alignments_V 5 -num_alignments_D 5 -num_alignments_J 5 -outfmt 3 -auxiliary_data optional_file/human_gl.aux -query /home/kfir/Pemphigus_TCR_Analysis/TAU260324/1/tracer_C2_exp78_S1_L001/cell/Trinity_output/cell_TCR_A.Trinity.fasta
BLAST query/options error: Germline annotation database human/human_TR_V could not be found in [internal_data] directory Please refer to the BLAST+ user manual.**
My IGDATA variable is set as /home/kfir/ncbi-igblast-1.22.0/bin.
And oddly enough this command runs with no errors when I set '-s Mmus'. But obviously, the results are of no use because my data is human.
What can be done to fix this? Am I the first one to encounter this behavior?