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TheDeanLab / navigate

navigate - open source light-sheet microscope controls
https://thedeanlab.github.io/navigate/
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Light sheet mode synchronization issue with Photometrics Iris 15 #899

Closed SJShep closed 3 months ago

SJShep commented 3 months ago

Cannot optimize remote focus parameters when using the Photometrics Iris 15 in light sheet mode. There are a few problems, which seem related to synchronization:

  1. Stopping and restarting acquire changes what we see with respect to the remote focusing.
    • image
    • Stop and start acquire: image
  2. After setting the parameters where the ASLM almost looks to be working, changing the exposure causes the current remote focus parameters to fail.
    • image
    • image

Camera Configuration image

annie-xd-wang commented 3 months ago

Hi @SJShep, I've made a fix that should solve problem 1. Please test it with the branch #899.

For the waveform parameters, you could try reducing the camera delay time to a shorter value, such as 1 ms. This parameter is set in the configuration.yaml file. At the same time, set an appropriate flyback value and settle-down time for the remote focus through the GUI.

SJShep commented 3 months ago

That fixed the problem with stopping and starting the acquisitions. image