Closed annie-xd-wang closed 1 month ago
I tested the code with this custom acquisition:
[{"name": ZStackAcquisition,},{"name": SetCameraParameters,"args": ("Light-Sheet","Bottom-to-Top",54,),},{"name": ZStackAcquisition,},]
Unfortunately, it was accompanied by a camera timeout. model_debug here:
2024-06-17 17:16:54,196 - model - DEBUG - common_features: *** Zstack move stage: (z: 10701.4), (f: -3139.1)
2024-06-17 17:16:54,231 - model - INFO - model: Navigate Model - Sent through pipe8
2024-06-17 17:16:54,495 - model - DEBUG - feature_container: running signal node: ZStackAcquisition
2024-06-17 17:16:54,496 - model - INFO - model: Navigate Model - Running data process, get frames [9]
2024-06-17 17:16:54,505 - model - DEBUG - feature_container: running signal node: SetCameraParameters
2024-06-17 17:16:54,537 - model - INFO - model: Navigate Model - Sent through pipe9
2024-06-17 17:16:55,399 - model - DEBUG - HamamatsuAPI: error message: -2147483386
2024-06-17 17:16:55,399 - model - INFO - model: Navigate Model - Running data process, get frames []
2024-06-17 17:16:55,399 - model - INFO - model: Navigate Model - Waiting 20
2024-06-17 17:16:55,400 - model - INFO - model: Navigate Model - Data thread stopped.
2024-06-17 17:16:55,400 - model - INFO - model: Navigate Model - Received frames in total: 10
It appears that the camera did switch modes, but it was not able to immediately begin imaging with the new settings thereafter...
I've tested it on CT-ASLM-V2, and this new feature is now working.
This will allow you to automatically switch camera parameters. For example, when switching from the mesoscale to the nanoscale modules on the multiscale microscope, the light-sheet readout direction could be adjusted. Or perhaps, in an automated routine, if the detected fluorescence is too dim, you could iteratively increase the exposure time.
The new feature can set sensor mode, readout direction, and the number of pixels for the rolling shutter.