Open blinard-BIOINFO opened 4 years ago
Hi, I'm trying to use tagdust on the simplest toy example I can imagine, but always get a core dump result. Can you tell me what I'm doing wrong ?
Thank you.
Command: ./tagdust -1 B:AACGCTTC -2 R:N sample.fasta -o test
./tagdust -1 B:AACGCTTC -2 R:N sample.fasta -o test
Input sequences (sample.fasta):
>M00234:812:000000000-J36YP:1:1102:17688:2041 1:N:0:GAGTGG AAAACGCTTCCTTCCGGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAGGAGAGTGACGGGCGGTGTGAAGCGTTG
As you can see, the barcode is present at the 3rd position of the input sequence. But I got this error:
$ ./tagdust -1 B:AACGCTTC -2 R:N sample.fasta -o test [2020-05-22 12:08:27] Tagdust 2.32, Copyright (C) 2013-2019 Timo Lassmann <timolassmann@gmail.com> [2020-05-22 12:08:27] cmd: ./tagdust -1 B:AACGCTTC -2 R:N -o test /home/ben/Dropbox/SPYGEN/DEMUX_TESTS/vsearc_merged_sample.fasta [2020-05-22 12:08:27] Start Run -------------------------------------------------- [2020-05-22 12:08:27] Determining threshold for read0. [2020-05-22 12:08:39] Long sequence found. Need to realloc model... [2020-05-22 12:09:13] Selected Threshold:: 0.513514 Segmentation fault (core dumped)
Hi, I'm trying to use tagdust on the simplest toy example I can imagine, but always get a core dump result. Can you tell me what I'm doing wrong ?
Thank you.
Command:
./tagdust -1 B:AACGCTTC -2 R:N sample.fasta -o test
Input sequences (sample.fasta):
As you can see, the barcode is present at the 3rd position of the input sequence. But I got this error: