TissueEngineeringLab / MyoFInDer

Python interface for automatic calculation of the fusion index in muscle cell cultures
https://tissueengineeringlab.github.io/MyoFInDer/
GNU General Public License v3.0
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Obvious nuclei not being detected #11

Closed WeisLeDocto closed 11 months ago

WeisLeDocto commented 11 months ago

MyoFInDer fails to detect some obvious nuclei on bright multi-channel images (see below the result of the computation). It is likely due to the filter in charge of removing the scale bar before computation (here in the code). As it is a bad practice to use images with a scale bar for analysis anyway, this feature will simply be removed. Users will have to correct manually the scale bar region if a scale bar is present and generates false positives.

794_P4_2-Image Export-02_c0-2x0-912y0-736 794_P4_2-Image Export-02_c0-2x0-912y0-736-processed