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TobiTekath / DTUrtle

Perform differential transcript usage (DTU) analysis of bulk or single-cell RNA-seq data. See documentation at:
https://tobitekath.github.io/DTUrtle
GNU General Public License v3.0
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barplot question #19

Closed m-noonan closed 7 months ago

m-noonan commented 1 year ago

Hello,

Something on my barplot seems off, like there are lots of thin, spread out lines. Is this okay?

Also, where can I find the "proportions" (mean proportion fit per subgroup (red line value) information used for this plotting?

plot_zoom_png plot_zoom_png-2

TobiTekath commented 7 months ago

Hi @m-noonan, I do not see an issue here. There should be one line for every cell / sample in your data - ordered by the fraction values of the first transcript on the left side of the plot. If there is a "blank" line, either the expression fraction is 0 - or the cell / sample has in general no expression of that gene, and therefore no fractions at all.

Hope this makes the visualization more clear.

m-noonan commented 7 months ago

@TobiTekath Yes this helps, thank you! Is there some way to condense it? Also, in regards to my second question before, where can I find the "proportions" (mean proportion fit per subgroup (red line value) information used for this plotting?