Trinotate / Trinotate.github.io

web documentation for Trinotate
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Which annotation tools do have an influence on GO term enrichment step? #11

Closed ghost closed 5 years ago

ghost commented 5 years ago

You recommend the following tools/DBs for annotation of assembled transcriptome:

Do I get it right, that only BLAST and hmmer results do have corresponding GO terms and hence have an influence on the later GO term enrichment analysis included in Trinity? I am asking this, because one could also assign GO terms for hits of RNAMMER, tmhmm etc. since this hints to a biological function. But I guess this is not implemented?

brianjohnhaas commented 5 years ago

Yes, all correct.

On Mon, Oct 22, 2018 at 11:11 AM MichaelsGITIGIT notifications@github.com wrote:

You recommend the following tools/DBs for annotation of assembled transcriptome:

  • BLAST using Swissprot
  • hmmer3 using Pfam database
  • SignalP
  • tmhmm
  • RNAMMER

Do I get it right, that only BLAST and hmmer results do have corresponding GO terms and hence have an influence on the later GO term enrichment analysis included in Trinity? I am asking this, because one could also assign GO terms for hits of RNAMMER, tmhmm etc. since this hints to a biological function. But I guess this is not implemented?

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