Trinotate SQlite error

[miguel1cifu]

Hey, I am fairly new to Bioinformatics, currently I am doing some Transcriptome reconstruction with Trinity and I want to use Trinotate to annotate the trinity sequences.

When I try to produce the sqlite database by inputing the code stated on the wiki: $TRINOTATE_HOME/admin/Build_Trinotate_Boilerplate_SQLite_db.pl Trinotate

I run into the following error code:

• Running CMD: /home/miguel/Descargas/Trinotate_Home/admin/util/EMBL_dat_to_Trinotate_sqlite_resourceDB.pl --sqlite Trinotate.sqlite --pfam Pfam-A.hmm.gz.pfam_sqlite_bulk_load CMD: echo "pragma journal_mode=memory; pragma synchronous=0; pragma cache_size=4000000; .mode tabs .import Pfam-A.hmm.gz.pfam_sqlite_bulk_load PFAMreference" | sqlite3 Trinotate.sqlite memory CREATE TABLE "PFAMreference"(...) failed: duplicate column name: 21.10 Error, cmd: echo "pragma journal_mode=memory; pragma synchronous=0; pragma cache_size=4000000; .mode tabs .import Pfam-A.hmm.gz.pfam_sqlite_bulk_load PFAMreference" | sqlite3 Trinotate.sqlite died with ret 256 at /home/miguel/Descargas/Trinotate_Home/admin/util/../../PerlLib/Sqlite_connect.pm line 190. Sqlite_connect::bulk_load_sqlite("Trinotate.sqlite", "PFAMreference", "Pfam-A.hmm.gz.pfam_sqlite_bulk_load") called at /home/miguel/Descargas/Trinotate_Home/admin/util/EMBL_dat_to_Trinotate_sqlite_resourceDB.pl line 109 Error, cmd: /home/miguel/Descargas/Trinotate_Home/admin/util/EMBL_dat_to_Trinotate_sqlite_resourceDB.pl --sqlite Trinotate.sqlite --pfam Pfam-A.hmm.gz.pfam_sqlite_bulk_load died with ret 256 at /home/miguel/Descargas/Trinotate_Home/admin/../PerlLib/Pipeliner.pm line 102. Pipeliner::run(Pipeliner=HASH(0x557d49d43458)) called at ../Trinotate_Home/admin/Build_Trinotate_Boilerplate_SQLite_db.pl line 120

As I said I don't have that much knowledge about code and any help would be very appreciated.

Thanks in advance