For a lot of our reads we find they have identical matches to multiple different tRFs and I think that means they are being counted twice in the analysis. I can't seem to figure out exactly what is done with such reads. Can you confirm if they are removed, counted twice or set to only one hit per read or something?
Hi,
For a lot of our reads we find they have identical matches to multiple different tRFs and I think that means they are being counted twice in the analysis. I can't seem to figure out exactly what is done with such reads. Can you confirm if they are removed, counted twice or set to only one hit per read or something?
Thanks, Conor