hi,
I'm having trouble using tRNAscan-SE.
input: tRNAscan-SE -B -o tRNA.out -f tRNA.ss -m tRNA.stats 001.fasta
error:
tRNAscan-SE v.2.0.9 (July 2021) - scan sequences for transfer RNAs
Copyright (C) 2020 Patricia Chan and Todd Lowe
University of California Santa Cruz
Freely distributed under the GNU General Public License (GPLv3)
Sequence file(s) to search: 001.fasta
Search Mode: Bacterial
Results written to: tRNA.out
Output format: Tabular
Searching with: Infernal First Pass->Infernal
Isotype-specific model scan: Yes
Covariance model: /public/home/wyang/miniconda3/envs/trna/lib/tRNAscan-SE/models/TRNAinf-bact.cm
/public/home/wyang/miniconda3/envs/trna/lib/tRNAscan-SE/models/TRNAinf-bact-SeC.cm
Infernal first pass cutoff score: 10
Temporary directory: /tmp
tRNA secondary structure
predictions saved to: tRNA.ss
Search statistics saved in: tRNA.stats
Status: Phase I: Searching for tRNAs with HMM-enabled Infernal
Status: Phase II: Infernal verification of candidate tRNAs detected with first-pass scan
substr outside of string at /public/home/wyang/miniconda3/envs/trna/lib/tRNAscan-SE/tRNAscanSE/Utils.pm line 117, line 16009.
hi, I'm having trouble using tRNAscan-SE. input: tRNAscan-SE -B -o tRNA.out -f tRNA.ss -m tRNA.stats 001.fasta error: tRNAscan-SE v.2.0.9 (July 2021) - scan sequences for transfer RNAs Copyright (C) 2020 Patricia Chan and Todd Lowe University of California Santa Cruz Freely distributed under the GNU General Public License (GPLv3)
Sequence file(s) to search: 001.fasta Search Mode: Bacterial Results written to: tRNA.out Output format: Tabular Searching with: Infernal First Pass->Infernal Isotype-specific model scan: Yes Covariance model: /public/home/wyang/miniconda3/envs/trna/lib/tRNAscan-SE/models/TRNAinf-bact.cm /public/home/wyang/miniconda3/envs/trna/lib/tRNAscan-SE/models/TRNAinf-bact-SeC.cm Infernal first pass cutoff score: 10
Temporary directory: /tmp tRNA secondary structure predictions saved to: tRNA.ss Search statistics saved in: tRNA.stats Status: Phase I: Searching for tRNAs with HMM-enabled Infernal Status: Phase II: Infernal verification of candidate tRNAs detected with first-pass scan substr outside of string at /public/home/wyang/miniconda3/envs/trna/lib/tRNAscan-SE/tRNAscanSE/Utils.pm line 117, line 16009.
How to solve it?
THANKS!