"Overall, we recommend the following polishing strategies:
Polypolish-careful alone when depth is very low (<5×), Polypolish-careful and Pypolcacareful when depth is low (5–25×), and Polypolish-default and Pypolca-careful when depth is
sufficient (>25×)."
https://www.biorxiv.org/content/10.1101/2024.03.07.584013v1.full.pdf
"Overall, we recommend the following polishing strategies: Polypolish-careful alone when depth is very low (<5×), Polypolish-careful and Pypolcacareful when depth is low (5–25×), and Polypolish-default and Pypolca-careful when depth is sufficient (>25×)."