VIB-PSB / MINI-EX

Motif-Informed Network Inference of cell type-specific gene regulatory networks in plants
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Error executing process > 'make_info_file (1)' #5

Closed qingzengLi closed 1 year ago

qingzengLi commented 2 years ago

Hi.I'm trying to use MINI-EX,but It report an error

N E X T F L O W  ~  version 22.04.0
Launching `miniex.nf` [amazing_varahamihira] DSL2 - revision: 7bd79d770f
      Motif-Informed Network Inference from gene EXpression
      =====================================================
Running TF motif enrichment filtering on  TF-F_motifs
      Running single-cell cluster enrichment using the top 700 upregulated genes per cluster
      Filtering out regulons of single-cell clusters where the TF is expressed in less than 10 % of the cells
Plotting expression specificity and DE calls for the top 150 regulons

executor >  local (10)
[2f/f17293] process > unzip_motifMappings      [100%] 1 of 1 ✔
[c4/9e17ed] process > run_enricher_motifs (1)  [100%] 1 of 1 ✔
executor >  local (10)
[2f/f17293] process > unzip_motifMappings      [100%] 1 of 1 ✔
[c4/9e17ed] process > run_enricher_motifs (1)  [100%] 1 of 1 ✔
[9c/92e903] process > filter_motifs (1)        [100%] 1 of 1 ✔
[ef/327f3c] process > get_topDEGs (1)          [100%] 1 of 1 ✔
[ea/da6a47] process > run_enricher_cluster (1) [100%] 1 of 1 ✔
[08/06ac5a] process > filter_expression (1)    [100%] 1 of 1 ✔
[62/071a24] process > make_info_file (1)       [100%] 1 of 1, failed: 1 ✘
[-        ] process > clustermap_regs (1)      -
[-        ] process > network_centrality (1)   -
[-        ] process > getFiles_enrichment (1)  -
[-        ] process > GO_enricher              -
[-        ] process > ranking_df_std           -
[-        ] process > makeBorda                -
[-        ] process > heatmap_tops             -
Error executing process > 'make_info_file (1)'

Caused by:
  Process `make_info_file (1)` terminated with an error exit status (127)
Command executed:

  python3 MINIEX_makeInfoFile.py "sample_matrix.txt" "sample_grnboost2.txt" "sample_enrichedRegulons.txt" "sample_regulons.txt" zma_TF2fam2mot.txt sample_cells2clusters.txt sample_identities.txt "sample_TF_info_file.txt"

Command exit status:
  127

Command output:
  (empty)

Command wrapper:
  .command.run: line 18: module: command not found

I don't know how to fix it.

qingzengLi commented 2 years ago

I install the Environment Modules and it release a new error:

Command wrapper:
  ERROR: Unable to locate a modulefile for 'python/x86_64/3.6.5'
caferra commented 2 years ago

Hi, and sorry for the late reply!

Have you checked in the work directory whether the input files are there? This is odd as all the input files of this process have already been used by previous processes.

I recently updated that python script, maybe try with that newer version.

I hope it helps!